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Sample GSM2028143 Query DataSets for GSM2028143
Status Public on Jan 07, 2016
Title HT29tsp53-p53 on- 1
Sample type RNA
 
Source name HT29-tsp53 38-32˚C (16h)
Organism Homo sapiens
Characteristics cell line: HT29-tsp53
cell type: Human Colorectal Adenocarcinoma Cell Line
genotype/variation: expressing a temperature sensitive variant of p53 (murine V135A p53 transgene)
temperature (38 or 32˚c): 38-32˚C
Treatment protocol One dish of subconfluent cells (60-70% confluent) were moved from the 37˚C incubator to a similarly regulated 32˚C incubator for 16 hours.
Growth protocol Cells were grown as adherent cultures in 5% CO2 at 37˚C in a humidified incubator.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Qiagen RNeasy Mini Kit according to the manufacturer's recommendations.
Label biotin
Label protocol 100ng of total RNA was labeled with Whole Transcript (WT) Sense Target Labeling kit as specified by the manufacturer (Affymetrix)
 
Hybridization protocol starndard Affymetrix protocol
Scan protocol Gene Chips were scanned with Affymetrix Scanner 3000.
Description expt 1 38 to 32
Data processing FlexArray 1.4.1, APT
probe group file: HuGene-1_0-st-v1.r3.pgf
meta-probeset file: HuGene-1_0-st-v1.r3.mps
 
Submission date Jan 06, 2016
Last update date Jan 07, 2016
Contact name Bruce McKay
E-mail(s) Bruce_McKay@carleton.ca
Phone 6135202600
Organization name Carleton University
Department Biology
Street address 1125 Colonel By Dr.
City Ottawa
State/province Ontario
ZIP/Postal code K1S 5B6
Country Canada
 
Platform ID GPL10739
Series (1)
GSE76575 The role of mRNA decay in p53-induced gene expression

Data table header descriptions
ID_REF
VALUE APT signal estimates from FlexArray 1.4.1

Data table
ID_REF VALUE
7892501 5.21844
7892502 3.93459
7892503 2.6831
7892504 7.32291
7892505 1.18597
7892506 3.97875
7892507 3.41355
7892508 4.33006
7892509 11.0578
7892510 3.69341
7892511 1.7926
7892512 5.16142
7892513 2.63611
7892514 9.58845
7892515 6.7869
7892516 5.18126
7892517 3.92441
7892518 2.49951
7892519 4.09557
7892520 7.92959

Total number of rows: 257430

Table truncated, full table size 4000 Kbytes.




Supplementary file Size Download File type/resource
GSM2028143_expt_1_38_to_32.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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