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Sample GSM2024797 Query DataSets for GSM2024797
Status Public on Nov 01, 2016
Title HEYA8_CELL, Biological rep. 1
Sample type RNA
 
Source name HEYA8 Cell
Organism Homo sapiens
Characteristics cell line: HeyA8
tissue type: Ovarian cancer
Treatment protocol N/A
Growth protocol All cells were maintained at 37°C with 5% CO2 and 95% air.
Extracted molecule total RNA
Extraction protocol Ovarian cancer cells were plated in cell culture medium containing 10% FBS and cultured for 24 h to 70% confluence. Media were replaced with 10% exosome-depleted FBS and cells were grown for 24hours more. Cells were collected by centrifugation and exosomes were isolated using supernatant with Total exosome isolation reagent (Invitrogen, Waltham, MA).
Label biotin
Label protocol Biotinylated polyA tailed RNA were prepared according to the standard Affymetrix protocol (FlashTag Biotin HSR RNA Labeling Kit, Affymetrix).
 
Hybridization protocol Following biotinylation, hybridization coctailed was prepared with 21.5ul labeled sample and incubated at 95C for minutes , then 45C for 5minutes. Following sample loading, arrays were loaded into trays and incibated at 48C abd 60rpm for 16 to 18hours.
Scan protocol The instructions for using the scanner can be found in Affymetrix Command Console User Guide.
Description Epithelial
miRNA
Data processing The data were analyzed with Expression Console. RMA + DABG analysis was performed for the normalization.
 
Submission date Dec 31, 2015
Last update date Nov 01, 2016
Contact name Gabriel Lopez-Berestein
E-mail(s) glopez@mdanderson.org
Organization name MD Anderson Cancer Center
Department Experimental Therapeutics
Lab Lopez Lab
Street address 1901 East Rd
City Houston
State/province TX
ZIP/Postal code 77054
Country USA
 
Platform ID GPL19117
Series (1)
GSE76449 miRNA Expression Data from Ovarian Cancer Exosomes and Cells of Origin

Data table header descriptions
ID_REF
VALUE Log2 RMA signal intensity

Data table
ID_REF VALUE
MIMAT0000062_st 12.22
MIMAT0004481_st 1.32
MIMAT0010195_st 7.44
MIMAT0000063_st 13.52
MIMAT0004482_st 3.22
MIMAT0000064_st 11.64
MIMAT0026472_st 1.75
MIMAT0000065_st 11.99
MIMAT0004484_st 2.84
MIMAT0000066_st 11.16
MIMAT0004485_st 3.98
MIMAT0000067_st 8.6
MIMAT0004486_st 2.41
MIMAT0004487_st 1.87
MIMAT0000068_st 8.92
MIMAT0004488_st 3.4
MIMAT0000069_st 12.46
MIMAT0004489_st 1.93
MIMAT0000070_st 13.96
MIMAT0000071_st 8.58

Total number of rows: 6658

Table truncated, full table size 134 Kbytes.




Supplementary file Size Download File type/resource
GSM2024797_03_HEYA8_1.CEL.gz 1.1 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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