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Sample GSM1975481 Query DataSets for GSM1975481
Status Public on Jan 27, 2016
Title PCLS_subject 4_INT747_rep2
Sample type RNA
Source name precision-cut liver slices, after 24h incubation with obeticholic acid (OCA; INT-747)
Organism Homo sapiens
Characteristics tissue: liver
treatment: 1 uM OCA (INT-747)
time point: 24h
patient_id: 4
gender: female
age (yr): 41
bmi: 37.7
Treatment protocol Liver slices were incubated in William's E Medium in 6-well plates at 37°C/5% CO2/80% O2 under continuous shaking (70 rpm). Duplicate wells were used per donor with 3 liver slices per well. After 1 hour, the media was replaced with fresh William's E Medium in the presence or absence of obeticholic acid (OCA, INT-747, 6α-ethyl-chenodeoxycholic acid) (1 μM) dissolved in dimethyl sulfoxide (DMSO, final concentration 0.1%). After 24 h incubation, liver slices were snap-frozen in liquid nitrogen and stored in -80°C for RNA isolation.
Growth protocol Liver biopsies were collected from obese patients undergoing bariatric surgery. After collection, biopsies were immediately placed in ice-cold oxygenated Belzer UW Cold Storage Solution and quickly transferred to the laboratory for further processing. 5 mm cylindrical liver cores were prepared with a surgical biopsy punch and sectioned to 200 μm slices using a Krumdieck tissue slicer.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from the liver slices using TRIzol reagent and purified using Qiagen RNeasy columns. RNA integrity was checked on chip analysis (Agilent 2100 Bioanalyzer, Agilent Technologies, Amsterdam, The Netherlands) according to the manufacturer's instructions. RNA was judged as suitable for array hybridization only if samples exhibited intact bands corresponding to the 18S and 28S ribosomal RNA subunits, and displayed no chromosomal peaks or RNA degradation products (RNA Integrity Number > 8.0).
Label biotin
Label protocol One hundred nanogram of RNA was used for whole transcript cDNA synthesis with the Ambion WT expression kit [catalog number 4411974] (Applied Biosystems/Life Technologies, Nieuwekerk a/d IJssel, The Netherlands).
Hybridization protocol Hybridization and washing of the Affymetrix GeneChip Human Gene 1.1 ST peg arrays were performed on a GeneTitan Instrument (Affymetrix, Santa Clara, CA) according to the manufacturer's recommendations.
Scan protocol Arrays were scanned on an Affymetrix GeneTitan instrument (Affymetrix, Santa Clara, CA).
Description G173_E11_14_4_INT.CEL
Data processing Expression estimates were calculated applying the RMA algorithm in the Bioconductor library 'Oligo' (v1.30.0).
Submission date Dec 18, 2015
Last update date Jan 27, 2016
Contact name Guido Hooiveld
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
Platform ID GPL11532
Series (2)
GSE76161 Gene expression profiling in human precision-cut liver slices upon treatment with the FXR agonist obeticholic acid [human]
GSE76163 Gene expression profiling in human precision-cut liver slices upon treatment with the FXR agonist obeticholic acid

Data table header descriptions
VALUE RMA signal (as log2)

Data table
7892501 6.600805466
7892502 5.102319923
7892503 3.647318725
7892504 8.359716886
7892505 1.43540064
7892506 4.654135014
7892507 4.738557526
7892508 6.730621667
7892509 10.747042
7892510 4.772233472
7892511 3.992205295
7892512 4.880329215
7892513 3.43909313
7892514 10.02927198
7892515 8.105981565
7892516 5.395292251
7892517 5.684461411
7892518 2.21843949
7892519 4.513387305
7892520 8.372150249

Total number of rows: 33297

Table truncated, full table size 646 Kbytes.

Supplementary file Size Download File type/resource
GSM1975481_G173_E11_14_4_INT.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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