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Sample GSM1971011 Query DataSets for GSM1971011
Status Public on Dec 29, 2020
Title CL1-5 cells expressing PSAT1 shRNA vector
Sample type RNA
 
Source name Lung adenocarcinoma
Organism Homo sapiens
Characteristics shRNA: PSAT1 shRNA vector
cell line: CL1-5
cell type: Type II alveolar cell
Treatment protocol Cell were selected with puromycin for two weeks. Stably transduced cells were use for this experiment.
Growth protocol Cells were cultured in 5% CO2 incubator at 37℃.
Extracted molecule total RNA
Extraction protocol Qiagene RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 PLUS 2.0 . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene expression data from CL1-5 cells expressing PSAT1 shRNA vector
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Dec 14, 2015
Last update date Dec 29, 2020
Contact name Michael Hsiao
E-mail(s) mhsiao@gate.sinica.edu.tw
Phone +886-2-2789-1243
Organization name Academia Sinica
Department Genomics Research Center
Street address Academia Road, Section 2, Nankang, Taipei, 115, Ta
City Taipei
ZIP/Postal code 049
Country Taiwan
 
Platform ID GPL570
Series (1)
GSE75961 Expression data from PSAT1 overexpression in CL1-0 lung cancer cell line and PSAT1 knockdown in CL1-5 lung cancer cell line.

Data table header descriptions
ID_REF
VALUE MAS 5.0 signal

Data table
ID_REF VALUE
AFFX-BioB-5_at 298.5239
AFFX-BioB-M_at 298.27615
AFFX-BioB-3_at 316.38348
AFFX-BioC-5_at 669.4326
AFFX-BioC-3_at 1187.4144
AFFX-BioDn-5_at 1689.2163
AFFX-BioDn-3_at 3634.0657
AFFX-CreX-5_at 9114.934
AFFX-CreX-3_at 12218.006
AFFX-DapX-5_at 9.689891
AFFX-DapX-M_at 11.254032
AFFX-DapX-3_at 11.413837
AFFX-LysX-5_at 7.280565
AFFX-LysX-M_at 10.33568
AFFX-LysX-3_at 7.494124
AFFX-PheX-5_at 9.604858
AFFX-PheX-M_at 8.5660095
AFFX-PheX-3_at 35.461964
AFFX-ThrX-5_at 18.450016
AFFX-ThrX-M_at 9.267632

Total number of rows: 54675

Table truncated, full table size 1090 Kbytes.




Supplementary file Size Download File type/resource
GSM1971011_CL1-5_shPSAT1.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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