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Sample GSM1971010 Query DataSets for GSM1971010
Status Public on Dec 29, 2020
Title CL1-5 cells expressing Non-silencing shRNA vector [NS]
Sample type RNA
 
Source name Lung adenocarcinoma
Organism Homo sapiens
Characteristics shRNA: non-silencing shRNA vector
cell line: CL1-5
cell type: Type II alveolar cell
Treatment protocol Cell were selected with puromycin for two weeks. Stably transduced cells were use for this experiment.
Growth protocol Cells were cultured in 5% CO2 incubator at 37℃.
Extracted molecule total RNA
Extraction protocol Qiagene RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 PLUS 2.0 . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene expression data from CL1-5 cells expressing non-silencing shRNA vector
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Dec 14, 2015
Last update date Dec 29, 2020
Contact name Michael Hsiao
E-mail(s) mhsiao@gate.sinica.edu.tw
Phone +886-2-2789-1243
Organization name Academia Sinica
Department Genomics Research Center
Street address Academia Road, Section 2, Nankang, Taipei, 115, Ta
City Taipei
ZIP/Postal code 049
Country Taiwan
 
Platform ID GPL570
Series (1)
GSE75961 Expression data from PSAT1 overexpression in CL1-0 lung cancer cell line and PSAT1 knockdown in CL1-5 lung cancer cell line.
Relations
Reanalysis of GSM1679472

Data table header descriptions
ID_REF
VALUE MAS 5.0 signal

Data table
ID_REF VALUE
AFFX-BioB-5_at 328.15735
AFFX-BioB-M_at 312.6235
AFFX-BioB-3_at 313.55606
AFFX-BioC-5_at 706.6095
AFFX-BioC-3_at 1203.8727
AFFX-BioDn-5_at 1708.645
AFFX-BioDn-3_at 3680.8867
AFFX-CreX-5_at 8966.784
AFFX-CreX-3_at 12114.741
AFFX-DapX-5_at 9.501167
AFFX-DapX-M_at 11.254032
AFFX-DapX-3_at 12.790451
AFFX-LysX-5_at 7.6788225
AFFX-LysX-M_at 9.82351
AFFX-LysX-3_at 7.494124
AFFX-PheX-5_at 9.308359
AFFX-PheX-M_at 7.880693
AFFX-PheX-3_at 34.19585
AFFX-ThrX-5_at 16.711252
AFFX-ThrX-M_at 11.115549

Total number of rows: 54675

Table truncated, full table size 1091 Kbytes.




Supplementary file Size Download File type/resource
GSM1971010_CL1-5_NS.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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