|
Status |
Public on Aug 25, 2016 |
Title |
HSPC_control rep1 |
Sample type |
RNA |
|
|
Source name |
Total RNA control HSPCs 4 days after pI:pC
|
Organism |
Mus musculus |
Characteristics |
background strain: B6/129
|
Treatment protocol |
Cells were FACS sorted from control and Med12Flox;MxCre mice 4 days after induction of Med12 deltion via pI:pC injection
|
Growth protocol |
Cells were directly isolated by FACS from mice
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNEasy Plus Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
|
Label |
biotin
|
Label protocol |
5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation, cDNA Biotin Module V2
|
|
|
Hybridization protocol |
3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
|
Scan protocol |
Affymetrix 7G GeneArray scanner
|
Description |
Gene expression data from Control Lineage negative, c-Kit and Sca1 (HSPCs) positive expressing bone marrow progenitors-repeat1
|
Data processing |
Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using geneChip program. No further adjustments were made to the data in the table.
|
|
|
Submission date |
Dec 10, 2015 |
Last update date |
Aug 25, 2016 |
Contact name |
Beatriz Aranda-Orgilles |
Organization name |
NYU School of Medicine
|
Street address |
522 First Avenue
|
City |
New York |
ZIP/Postal code |
10016 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE75879 |
Expression data from control and Med12-deficient hematopoietic stem cells and progenitors |
GSE76055 |
Regulation of enhancer dynamics by MED12 |
|