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Sample GSM1964022 Query DataSets for GSM1964022
Status Public on May 01, 2016
Title Burn-1
Sample type SRA
Source name Burn injury_DRG neurons
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: wild type
gender: male
age: 6-8 weeks
sample group: three days after the burn injury
tissue: L3, L4, L5 whole DRG neurons
Extracted molecule total RNA
Extraction protocol Whole DRGs were removed and immediately put into RNALater and cut into pieces in RNA later. RNA was extracted using the Rneasy Mini kit (Qiagen)
RNA libraries were prepared using TruSeq stranded total RNA library preparation.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Data processing Alignment: STAR aligner (version star/2.3.0e) and samtools
Mapping: Mapped to Ensembl Mus musculus mm10 genome (version GRCm38)
Counts: Generated using HTSeq_count in the HTSeq package
Genome_build: mm10
Supplementary_files_format_and_content: .txt files, tab-delimited.
Submission date Dec 03, 2015
Last update date May 15, 2019
Contact name Kathleen Yin
Organization name University of Queensland
Department Institute of Molecular Biosciences
Street address 306 Carmody Rd
City St Lucia
State/province Queensland
ZIP/Postal code 4067
Country Australia
Platform ID GPL19057
Series (1)
GSE75691 Transcriptomic and behavioural characterisation of a mouse model of burns pain
BioSample SAMN04316864
SRA SRX1462882

Supplementary file Size Download File type/resource 146.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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