|
Status |
Public on Jun 01, 2016 |
Title |
Plasmid_replicate_3_79k_Pool |
Sample type |
SRA |
|
|
Source name |
plasmid DNA
|
Organism |
synthetic construct |
Characteristics |
cell line: Plasmid DNA
|
Growth protocol |
24 hour growth post-transfection
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Qiagen Rneasy followed by hybridization pulldown of the GFP transcript. Illumina Custom Paired-end (TruSeq)
|
|
|
Library strategy |
OTHER |
Library source |
genomic |
Library selection |
other |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
79k Oligo Pool barcode count data fastq of 3' UTR barcode and residual 5' flanking sequencing
|
Data processing |
Library strategy: MPRA Perfect UTR barcode matches were asigned to their respective oligo. Counts for all barcodes asigned to an oligo were summed. Genome_build: hg19
|
|
|
Submission date |
Dec 03, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Ryan Tewhey |
Organization name |
Jackson Laboratory
|
Street address |
600 Main Street
|
City |
Bar Harbor |
State/province |
ME |
ZIP/Postal code |
04609 |
Country |
USA |
|
|
Platform ID |
GPL19604 |
Series (1) |
GSE75661 |
Direct identification of hundreds of expression-modulating variants using a multiplexed reporter assay |
|
Relations |
BioSample |
SAMN04313650 |
SRA |
SRX1462148 |