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Sample GSM1962996 Query DataSets for GSM1962996
Status Public on Jun 01, 2016
Title Plasmid_replicate_2_79k_Pool
Sample type SRA
Source name plasmid DNA
Organism synthetic construct
Characteristics cell line: Plasmid DNA
Growth protocol 24 hour growth post-transfection
Extracted molecule genomic DNA
Extraction protocol Qiagen Rneasy followed by hybridization pulldown of the GFP transcript.
Illumina Custom Paired-end (TruSeq)
Library strategy OTHER
Library source genomic
Library selection other
Instrument model Illumina HiSeq 2500
Description 79k Oligo Pool
barcode count data
fastq of 3' UTR barcode and residual 5' flanking sequencing
Data processing Library strategy: MPRA
Perfect UTR barcode matches were asigned to their respective oligo. Counts for all barcodes asigned to an oligo were summed.
Genome_build: hg19
Submission date Dec 03, 2015
Last update date May 15, 2019
Contact name Ryan Tewhey
Organization name Jackson Laboratory
Street address 600 Main Street
City Bar Harbor
State/province ME
ZIP/Postal code 04609
Country USA
Platform ID GPL19604
Series (1)
GSE75661 Direct identification of hundreds of expression-modulating variants using a multiplexed reporter assay
BioSample SAMN04313649
SRA SRX1462147

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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