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Status |
Public on Jun 01, 2016 |
Title |
Lockd Control, rep 1 |
Sample type |
RNA |
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|
Source name |
G1E_Lockd control
|
Organism |
Mus musculus |
Characteristics |
cell line background: G1E cell type: erythroid cell line genotype/variation: Lockd WT
|
Treatment protocol |
CRISPR/Cas9 was used to delete Lockd
|
Growth protocol |
Cells were grown in G1E media as described previously (Weiss et al, MCB 1997)
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated from cultured cells using the Qiagen Rneasy kit
|
Label |
biotin
|
Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
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|
|
Hybridization protocol |
Samples were hybridized with GeneChip Mogene 2.0 ST Arrays (Affymetrix) and scanned at the University of Pennsylvania Molecular Profiling Facility
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Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
Description |
WT.U.1
|
Data processing |
Raw data were normalized useing RMA algorithm (rma-gene-full option). Data analysis and statistical evaluations were performed with customized Matlab scripts (R2012b) affymetrix-algorithm-name = rma-gene-full affymetrix-algorithm-version = 1.0 program-name = Expression Console program-version = 1.3.1.187
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Submission date |
Nov 27, 2015 |
Last update date |
Jun 01, 2016 |
Contact name |
Vikram Paralkar |
E-mail(s) |
vikram.paralkar@uphs.upenn.edu
|
Phone |
215-590-0558
|
Organization name |
University of Pennsylvania
|
Street address |
3400 Civic Center Boulevard, Perelman Center, 7th Floor, South Pavilion Extension
|
City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19104 |
Country |
USA |
|
|
Platform ID |
GPL16570 |
Series (2) |
GSE75449 |
Unlinking a lncRNA from its associated cis element [gene expression] |
GSE75881 |
Unlinking a lncRNA from its associated cis element |
|