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Sample GSM1954974 Query DataSets for GSM1954974
Status Public on Nov 27, 2015
Title IPJ2015_007
Sample type SRA
 
Source name Monocytes
Organism Homo sapiens
Characteristics cell type: Monocytes
disease state: healthy
source: Peripheral blood mononuclear cells
Extracted molecule total RNA
Extraction protocol RNA was harvested using Trizol reagent. Ion AmpliSeqâ„¢ Transcriptome Human Gene Expression Kit (#A26325) was used with 1 ng of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Ion torrent protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Ion Torrent Proton
 
Data processing The Ion Proton reads were analyzed using the AmpliSeqRNA analysis plugin, v4.2.1, in the Torrent Suite Software (Life Technologies)
Reads per million reads (RPM):Counts the number of sequences obtained for all cDNA amplicons
Supplementary_files_format_and_content: tab-delimited text files include RPM values for each Sample
 
Submission date Nov 26, 2015
Last update date May 15, 2019
Contact name Kosuke Kawaguchi
E-mail(s) kkosuke@kuhp.kyoto-u.ac.jp
Organization name Kyoto University
Street address 54 Kawaharacho Shogoin Sakyo-ku
City Kyoto
ZIP/Postal code 6068075
Country Japan
 
Platform ID GPL17303
Series (1)
GSE75428 Downregulation of neuropilin-1 on macrophages modulates antibody-mediated tumoricidal activity
Relations
BioSample SAMN04296877
SRA SRX1452938

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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