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Sample GSM1954909 Query DataSets for GSM1954909
Status Public on Oct 12, 2016
Title NESCs_12d_rep4
Sample type RNA
Source name Proliferative phase normal endometrium, 12 days, replicate4
Organism Homo sapiens
Characteristics cell type: proliferative phase normal endometrium
gender: Female
age: 41y
treatment: 12d 10% charcoal-stripped heat-inactivated FBS
Treatment protocol NESCs were obtained from premenopausal patients who had undergone hysterectomies for leiomyoma and had no evidence of endometriosis. NESCs were isolated from the corresponding tissues by enzymatic digestion. NESCs were cultured in DMEM supplemented with 100 IU/ml of penicillin, 50 mg/ml of streptomycin, and 10% charcoal-stripped heat-inactivated fetal FBS at 37°C in 5% CO2 in air.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from tissues using TRIzol Reagent (nitrogen) and purified using SV Total RNA Isolation System (Promega)
Label Cy3
Label protocol cRNA was amplified and labelled using a Low input Quick Amp Labelling Kit (Agilent Technologies).
Hybridization protocol cRNA was hybridized to a 60K 60-mer oligomicroarray (SurePrint G3 Human miRNA Microarray, Release 19.0, 8x60K ; Agilent Technologies) according to the manufacturer's instructions.
Scan protocol The hybridized microarray slides were scanned using an Agilent scanner. The relative hybridization intensities and background hybridization values were calculated using Feature Extraction Software version (Agilent Technologies).
Description miRNA expression after 12d_10% charcoal-stripped heat-inactivated FBS
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. The raw signal intensities and flags for each probe were calculated from the hybridization intensities and spot information according to the procedures recommended by Agilent Technologies using the Flag criteria in the GeneSpring Software. The raw signal intensities of all samples were normalized by the quantile algorithm with the Bioconductor.
Submission date Nov 25, 2015
Last update date Oct 12, 2016
Contact name yoko aoyagi
Organization name oita university
Street address 1-1 Idaigaoka hamasamachi
City yufu city
ZIP/Postal code 879-5593
Country Japan
Platform ID GPL18402
Series (2)
GSE75424 miRNA expression profiles in decidualized and non-decidualized normal endometrial stromal cells (NESCs).
GSE75427 Expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs) and normal endometrial stromal cells (NESCs)

Data table header descriptions
VALUE quantile normalized signal, non-log scaled and ABS CALL.

Data table
hsa-let-7a-3p 0.1 Not Detected
hsa-let-7a-5p 5634.994513 Detected
hsa-let-7b-3p 0.1 Not Detected
hsa-let-7b-5p 4364.28375 Detected
hsa-let-7c 1073.19698 Detected
hsa-let-7d-3p 3.9570075 Detected
hsa-let-7d-5p 518.6296413 Detected
hsa-let-7e-3p 0.1 Not Detected
hsa-let-7e-5p 641.6630425 Detected
hsa-let-7f-1-3p 0.1 Not Detected
hsa-let-7f-2-3p 0.1 Not Detected
hsa-let-7f-5p 2665.162088 Detected
hsa-let-7g-3p 0.1 Not Detected
hsa-let-7g-5p 980.87124 Detected
hsa-let-7i-3p 0.1 Not Detected
hsa-let-7i-5p 1734.980963 Detected
hsa-miR-1 0.1 Not Detected
hsa-miR-100-3p 0.1 Not Detected
hsa-miR-100-5p 96.8180275 Detected
hsa-miR-101-3p 38.54780875 Detected

Total number of rows: 2006

Table truncated, full table size 62 Kbytes.

Supplementary file Size Download File type/resource
GSM1954909_US11030397_254606412148_S01_miRNA_107_Sep09_1_4.txt.gz 8.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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