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Sample GSM1954902 Query DataSets for GSM1954902
Status Public on Oct 12, 2016
Title ECSCs_12d_dibutyryl-cAMP and dienogest_rep1
Sample type RNA
Source name endometriotic cyst stromal cells, 12 days, dibutyryl-cAMP and dienogest, replicate1
Organism Homo sapiens
Characteristics cell type: endometriotic cyst stromal cells
gender: Female
age: 34y
treatment: 12d dibutyryl-cAMP and dienogest
Treatment protocol ECSCs were obtained from premenopausal patients who had undergone salpingo-oophorectomy or cystectomy for ovarian endometriotic cysts. ECSCs were isolated from the corresponding tissues by enzymatic digestion. ECSCs were cultured in DMEM supplemented with 100 IU/ml of penicillin, 50 mg/ml of streptomycin, and 10% charcoal-stripped heat-inactivated fetal FBS at 37°C in 5% CO2 in air.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from tissues using TRIzol Reagent (nitrogen) and purified using SV Total RNA Isolation System (Promega)
Label Cy3
Label protocol cRNA was amplified and labelled using a Low input Quick Amp Labelling Kit (Agilent Technologies).
Hybridization protocol cRNA was hybridized to a 60K 60-mer oligomicroarray (SurePrint G3 Human Gene Expression Microarray 8x60K v2; Agilent Technologies) according to the manufacturer's instructions.
Scan protocol The hybridized microarray slides were scanned using an Agilent scanner. The relative hybridization intensities and background hybridization values were calculated using Feature Extraction Software version (Agilent Technologies).
Description mRNA expression after 12d_dibutyryl-cAMP and dienogest
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. The raw signal intensities and flags for each probe were calculated from the hybridization intensities and spot information according to the procedures recommended by Agilent Technologies using the Flag criteria in the GeneSpring Software. The raw signal intensities of all samples were normalized by the quantile algorithm with the Bioconductor.
Submission date Nov 25, 2015
Last update date Oct 12, 2016
Contact name yoko aoyagi
Organization name oita university
Street address 1-1 Idaigaoka hamasamachi
City yufu city
ZIP/Postal code 879-5593
Country Japan
Platform ID GPL13497
Series (2)
GSE75423 mRNA expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs).
GSE75427 Expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs) and normal endometrial stromal cells (NESCs)

Data table header descriptions
VALUE quantile normalized signal, non-log scaled and ABS CALL.

Data table
A_23_P100001 1994.738375 P
A_23_P100022 6.53810275 A
A_23_P100056 3.500292875 A
A_23_P100074 5435.946026 P
A_23_P100127 21.27422684 M
A_23_P100141 639.1587066 P
A_23_P100189 10.14711543 M
A_23_P100196 593.3750671 P
A_23_P100203 2253.099591 P
A_23_P100220 28.63802526 P
A_23_P100240 3.501637125 A
A_23_P10025 11.9946963 M
A_23_P100292 9017.237386 P
A_23_P100315 1763.965375 P
A_23_P100326 4459.525875 P
A_23_P100344 116.5427503 P
A_23_P100355 1091.475625 P
A_23_P100386 3.811475478 A
A_23_P100392 1201.85205 P
A_23_P100420 1336.983924 P

Total number of rows: 34127

Table truncated, full table size 885 Kbytes.

Supplementary file Size Download File type/resource
GSM1954902_252665215262_S01_GE1_107_Sep09_1_4.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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