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Sample GSM1954894 Query DataSets for GSM1954894
Status Public on Oct 12, 2016
Title ECSCs_12d_dibutyryl-cAMP and dienogest_rep1
Sample type RNA
 
Source name endometriotic cyst stromal cells, 12 days, dibutyryl-cAMP and dienogest, replicate1
Organism Homo sapiens
Characteristics gender: Female
age: 34y
treatment: 12d dibutyryl-cAMP and dienogest
Treatment protocol ECSCs were obtained from premenopausal patients who had undergone salpingo-oophorectomy or cystectomy for ovarian endometriotic cysts. ECSCs were isolated from the corresponding tissues by enzymatic digestion. ECSCs were cultured in DMEM supplemented with 100 IU/ml of penicillin, 50 mg/ml of streptomycin, and 10% charcoal-stripped heat-inactivated fetal FBS at 37°C in 5% CO2 in air.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from tissues using TRIzol Reagent (nitrogen) and purified using SV Total RNA Isolation System (Promega)
Label Cy3
Label protocol cRNA was amplified and labelled using a Low input Quick Amp Labelling Kit (Agilent Technologies).
 
Hybridization protocol cRNA was hybridized to a 60K 60-mer oligomicroarray (SurePrint G3 Human miRNA Microarray, Release 19.0, 8x60K ; Agilent Technologies) according to the manufacturer's instructions.
Scan protocol The hybridized microarray slides were scanned using an Agilent scanner. The relative hybridization intensities and background hybridization values were calculated using Feature Extraction Software version 9.5.1.1 (Agilent Technologies).
Description miRNA expression after 12d_dibutyryl-cAMP and dienogest
Data processing The scanned images were analyzed with Feature Extraction Software 9.5.1.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. The raw signal intensities and flags for each probe were calculated from the hybridization intensities and spot information according to the procedures recommended by Agilent Technologies using the Flag criteria in the GeneSpring Software. The raw signal intensities of all samples were normalized by the quantile algorithm with the Bioconductor.
 
Submission date Nov 25, 2015
Last update date Oct 12, 2016
Contact name yoko aoyagi
E-mail(s) yokoao@oita-u.ac.jp
Organization name oita university
Street address 1-1 Idaigaoka hamasamachi
City yufu city
ZIP/Postal code 879-5593
Country Japan
 
Platform ID GPL18402
Series (2)
GSE75422 miRNA expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs).
GSE75427 Expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs) and normal endometrial stromal cells (NESCs)

Data table header descriptions
ID_REF
VALUE quantile normalized signal, non-log scaled and ABS CALL.
CALL

Data table
ID_REF VALUE CALL
hsa-let-7a-3p 0.1 Not Detected
hsa-let-7a-5p 9443.42895 Detected
hsa-let-7b-3p 0.1 Not Detected
hsa-let-7b-5p 6190.017538 Detected
hsa-let-7c 1488.791625 Detected
hsa-let-7d-3p 0.1 Not Detected
hsa-let-7d-5p 663.6933788 Detected
hsa-let-7e-3p 0.1 Not Detected
hsa-let-7e-5p 1087.205425 Detected
hsa-let-7f-1-3p 0.1 Not Detected
hsa-let-7f-2-3p 0.1 Not Detected
hsa-let-7f-5p 5235.385563 Detected
hsa-let-7g-3p 0.1 Not Detected
hsa-let-7g-5p 1133.558228 Detected
hsa-let-7i-3p 0.1 Not Detected
hsa-let-7i-5p 2625.399588 Detected
hsa-miR-1 0.1 Not Detected
hsa-miR-100-3p 2.87486375 Detected
hsa-miR-100-5p 3374.211013 Detected
hsa-miR-101-3p 66.70834625 Detected

Total number of rows: 2006

Table truncated, full table size 62 Kbytes.




Supplementary file Size Download File type/resource
GSM1954894_254606410949_S01_miRNA_107_Sep09_1_1.txt.gz 8.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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