|
| Status |
Public on Oct 12, 2016 |
| Title |
ECSCs_12d_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
endometriotic cyst stromal cells, 12 days, replicate4
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
age: 28y
treatment: 12d 10% charcoal-stripped heat-inactivated FBS
|
| Treatment protocol |
ECSCs were obtained from premenopausal patients who had undergone salpingo-oophorectomy or cystectomy for ovarian endometriotic cysts. ECSCs were isolated from the corresponding tissues by enzymatic digestion. ECSCs were cultured in DMEM supplemented with 100 IU/ml of penicillin, 50 mg/ml of streptomycin, and 10% charcoal-stripped heat-inactivated fetal FBS at 37°C in 5% CO2 in air.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from tissues using TRIzol Reagent (nitrogen) and purified using SV Total RNA Isolation System (Promega)
|
| Label |
Cy3
|
| Label protocol |
cRNA was amplified and labelled using a Low input Quick Amp Labelling Kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
cRNA was hybridized to a 60K 60-mer oligomicroarray (SurePrint G3 Human miRNA Microarray, Release 19.0, 8x60K ; Agilent Technologies) according to the manufacturer's instructions.
|
| Scan protocol |
The hybridized microarray slides were scanned using an Agilent scanner. The relative hybridization intensities and background hybridization values were calculated using Feature Extraction Software version 9.5.1.1 (Agilent Technologies).
|
| Description |
miRNA expression after 12d_10% charcoal-stripped heat-inactivated FBS
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.5.1.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. The raw signal intensities and flags for each probe were calculated from the hybridization intensities and spot information according to the procedures recommended by Agilent Technologies using the Flag criteria in the GeneSpring Software. The raw signal intensities of all samples were normalized by the quantile algorithm with the Bioconductor.
|
| |
|
| Submission date |
Nov 25, 2015 |
| Last update date |
Oct 12, 2016 |
| Contact name |
yoko aoyagi |
| E-mail(s) |
yokoao@oita-u.ac.jp
|
| Organization name |
oita university
|
| Street address |
1-1 Idaigaoka hamasamachi
|
| City |
yufu city |
| ZIP/Postal code |
879-5593 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18402 |
| Series (2) |
| GSE75422 |
miRNA expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs). |
| GSE75427 |
Expression profiles in decidualized and non-decidualized endometriotic cyst stromal cells (ECSCs) and normal endometrial stromal cells (NESCs) |
|
