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Sample GSM195326 Query DataSets for GSM195326
Status Public on Oct 02, 2007
Title Melanoma metastatic cell line A375, P7, biol rep7 (LeiFidler)
Sample type RNA
 
Source name A375 parental cell line
Organism Homo sapiens
Characteristics Genotype: poorly metastatic
Age: none
Treatment protocol n/a
Growth protocol n/a
Extracted molecule total RNA
Extraction protocol Rneasy mini kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol (701025, Revision 3).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized on one U133A human chip and one test chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description human melanoma metastatic cell line A375
Data processing raw data (CEL file) were normalized with RMA and further analyzied with GenePatter comparative marker selection module.
 
Submission date May 30, 2007
Last update date Dec 30, 2011
Contact name steve shen
E-mail(s) ssshen@mit.edu
Organization name MIT
Street address 77 mass ave
City cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platform ID GPL96
Series (1)
GSE7956 Gene Signature for Aggression of Melanoma Metastases - Melanoma Metastasis (LeiFidler)

Data table header descriptions
ID_REF
VALUE RMA normalized

Data table
ID_REF VALUE
1007_s_at 1357.932946
1053_at 691.40647
117_at 159.553045
121_at 885.089034
1255_g_at 31.691685
1294_at 138.96258
1316_at 62.68907
1320_at 47.983066
1405_i_at 31.571085
1431_at 30.438148
1438_at 325.764723
1487_at 353.390621
1494_f_at 177.390704
1598_g_at 484.993925
160020_at 690.914712
1729_at 354.665071
1773_at 392.58804
177_at 78.285958
179_at 870.440378
1861_at 148.514043

Total number of rows: 22283

Table truncated, full table size 468 Kbytes.




Supplementary file Size Download File type/resource
GSM195326.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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