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Sample GSM195324 Query DataSets for GSM195324
Status Public on Oct 02, 2007
Title Melanoma metastatic cell line A375, P5, biol rep5 (LeiFidler)
Sample type RNA
 
Source name A375 parental cell line
Organism Homo sapiens
Characteristics Genotype: poorly metastatic
Age: none
Treatment protocol n/a
Growth protocol n/a
Extracted molecule total RNA
Extraction protocol Rneasy mini kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol (701025, Revision 3).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized on one U133A human chip and one test chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description human melanoma metastatic cell line A375
Data processing raw data (CEL file) were normalized with RMA and further analyzied with GenePatter comparative marker selection module.
 
Submission date May 30, 2007
Last update date Dec 30, 2011
Contact name steve shen
E-mail(s) ssshen@mit.edu
Organization name MIT
Street address 77 mass ave
City cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platform ID GPL96
Series (1)
GSE7956 Gene Signature for Aggression of Melanoma Metastases - Melanoma Metastasis (LeiFidler)

Data table header descriptions
ID_REF
VALUE RMA normalized

Data table
ID_REF VALUE
1007_s_at 1400.826934
1053_at 768.350665
117_at 170.248468
121_at 939.251102
1255_g_at 33.171537
1294_at 130.706059
1316_at 86.179346
1320_at 47.330093
1405_i_at 31.818602
1431_at 32.360499
1438_at 331.726064
1487_at 418.090671
1494_f_at 180.538098
1598_g_at 571.578911
160020_at 631.769371
1729_at 357.672173
1773_at 241.296703
177_at 82.78227
179_at 901.703784
1861_at 176.500724

Total number of rows: 22283

Table truncated, full table size 468 Kbytes.




Supplementary file Size Download File type/resource
GSM195324.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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