|
| Status |
Public on Oct 02, 2007 |
| Title |
Melanoma metastatic cell line A375, P4, biol rep4 (LeiFidler) |
| Sample type |
RNA |
| |
|
| Source name |
A375 parental cell line
|
| Organism |
Homo sapiens |
| Characteristics |
Genotype: poorly metastatic
Age: none
|
| Treatment protocol |
n/a
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy mini kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (701025, Revision 3).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized on one U133A human chip and one test chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
human melanoma metastatic cell line A375
|
| Data processing |
raw data (CEL file) were normalized with RMA and further analyzied with GenePatter comparative marker selection module.
|
| |
|
| Submission date |
May 30, 2007 |
| Last update date |
Dec 30, 2011 |
| Contact name |
steve shen |
| E-mail(s) |
ssshen@mit.edu
|
| Organization name |
MIT
|
| Street address |
77 mass ave
|
| City |
cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE7956 |
Gene Signature for Aggression of Melanoma Metastases - Melanoma Metastasis (LeiFidler) |
|
