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Sample GSM1947376 Query DataSets for GSM1947376
Status Public on Jun 28, 2016
Title GTPP sample 3
Sample type SRA
 
Source name HeLa cells
Organism Homo sapiens
Characteristics cell line: HeLa
Extracted molecule total RNA
Extraction protocol NucleoSpin miRNA (Macherey-Nagel)
ribosomal depletion with RiboZero, directional RNA-seq library preparation, and 12 cycle amplification using LongAmp (New England BioLabs) and indexed primers (Integrated DNA Technologies)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Data processing FastQC
tophat2 v1.2 data analysis pipeline at HMS research computing
tophat vs hg19
cufflinks
cuffmerge
cuffdiff
Genome_build: hg19
Supplementary_files_format_and_content: text delimited file containing FPKM values for each sample, fold-changes with p-values, and sequecinging status
 
Submission date Nov 20, 2015
Last update date May 15, 2019
Contact name Christian Munch
E-mail(s) christian_munch@hms.harvard.edu
Organization name Harvard Medical School
Department Cell Biology
Street address 240 Longwood Avenue
City Boston
ZIP/Postal code 02115
Country USA
 
Platform ID GPL18573
Series (2)
GSE75247 Mitochondrial unfolded protein response controls matrix pre-RNA processing and translation
GSE75411 Mitochondrial unfolded protein response controls matrix pre-RNA processing and translation
Relations
BioSample SAMN04283930
SRA SRX1441552

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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