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Status
Public on Feb 20, 2016
Title
B34BM_3
Sample type
RNA
Source name
hFOB
Organism
Homo sapiens
Characteristics
cell line: hFOB treatment: untreated, grow at 34oC in BM
Treatment protocol
BM, basal madia: DMEM supplemented 10% calf serum, 2mM L-glutamine, 1mM sodium pyruvate, 1X non-essential amino acids, and 1% penicillin-streptomycin
Growth protocol
BM, basal madia: DMEM supplemented 10% calf serum, 2mM L-glutamine, 1mM sodium pyruvate, 1X non-essential amino acids, and 1% penicillin-streptomycin
Extracted molecule
total RNA
Extraction protocol
none provided by submitter
Label
biotin
Label protocol
standard Affymetrix protocol
Hybridization protocol
standard Affymetrix protocol
Scan protocol
standard Affymetrix protocol
Description
B34BM_3
Data processing
Affymetrix human Genome U133 2.0 array data in CEL files were preprocessed using Affymetrix Expression Console software with mas5 and its quantile normalization method. Mean expression values from biological duplicates were calculated.
Submission date
Nov 20, 2015
Last update date
Feb 20, 2016
Contact name
Songjoon Baek
Organization name
NCI / NIH
Department
CCR
Lab
LRBGE
Street address
41 Library Drive
City
Bethesda
State/province
MD
ZIP/Postal code
20892
Country
USA
Platform ID
GPL570
Series (2)
GSE75230
Genome-Wide Chromatin Landscape Transitions Identify Novel Pathways in Early Commitment to Osteoblast Differentiation (microarray)
GSE75232
Genome-Wide Chromatin Landscape Transitions Identify Novel Pathways in Early Commitment to Osteoblast Differentiation
Data table header descriptions
ID_REF
VALUE
Signal
ABS_CALL
indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
416.863
P
6.02111e-05
AFFX-BioB-M_at
584.665
P
4.42873e-05
AFFX-BioB-3_at
353.685
P
4.42873e-05
AFFX-BioC-5_at
944.703
P
5.16732e-05
AFFX-BioC-3_at
1662.45
P
4.42873e-05
AFFX-BioDn-5_at
2585.69
P
4.42873e-05
AFFX-BioDn-3_at
4300.84
P
5.16732e-05
AFFX-CreX-5_at
11394.7
P
5.16732e-05
AFFX-CreX-3_at
11653.6
P
4.42873e-05
AFFX-DapX-5_at
99.3441
P
0.000224668
AFFX-DapX-M_at
369.438
P
0.000662269
AFFX-DapX-3_at
607.49
P
4.42873e-05
AFFX-LysX-5_at
46.477
P
0.000662269
AFFX-LysX-M_at
41.2538
P
0.00618711
AFFX-LysX-3_at
102.59
P
6.02111e-05
AFFX-PheX-5_at
42.7389
P
0.000146581
AFFX-PheX-M_at
81.2903
P
0.00159257
AFFX-PheX-3_at
88.7755
P
7.00668e-05
AFFX-ThrX-5_at
22.9117
A
0.083592
AFFX-ThrX-M_at
48.5055
P
0.000753643
Total number of rows: 54675 Table truncated, full table size 1640 Kbytes .Supplementary file
Size
Download
File type/resource
GSM1946414_B34BM_3.CEL.gz
4.6 Mb
(ftp) (http)
CEL
GSM1946414_B34BM_3.mas5.CHP.gz
487.0 Kb
(ftp) (http)
CHP
Processed data included within Sample table
Processed data provided as supplementary file