|
| Status |
Public on Feb 12, 2018 |
| Title |
Ezh2 fl/fl Runx1 fl/fl mouse Mac1+ cells rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Mac1+ cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: Ezh2 fl/fl Runx1 fl/fl
|
| Extracted molecule |
total RNA |
| Extraction protocol |
100 cells were FACS-sorted directly into lysis buffer
Picelli et al, Nature Protocols 2014: Full-length RNA-seq from single cells using Smart-seq2.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
386-WT_S5
|
| Data processing |
Alignment to mm10 transcriptome with STAR (version 2.4.2a)
Merge Technical replicates with samtools (version 1.1)
Filter non-uniquely mapped reads with samtools (version 1.1)
Count Reads in transcripts with Htseq-count (version 0.6.1p1)
Genome_build: mm10
Supplementary_files_format_and_content: RPKM values
|
| |
|
| Submission date |
Nov 19, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Adam Mead |
| E-mail(s) |
adam.mead@imm.ox.ac.uk
|
| Organization name |
University of Oxford
|
| Department |
Weatherall Institute of Molecular Medicine
|
| Lab |
HSCB Laboratory
|
| Street address |
John Radcliffe Hospital
|
| City |
Oxford |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE75187 |
Ezh2 and Runx1 Mutations Targeted to Early Lymphoid Progenitors Collaborate to Promote Early Thymic Progenitor Leukemia [RNA-Seq 2] |
| GSE75188 |
Ezh2 and Runx1 Mutations Targeted to Early Lymphoid Progenitors Collaborate to Promote Early Thymic Progenitor Leukemia |
|
| Relations |
| BioSample |
SAMN04279019 |
| SRA |
SRX1438379 |
