NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1944738 Query DataSets for GSM1944738
Status Public on Feb 12, 2018
Title Ezh2 fl/fl Runx1 fl/fl Rag1Cre+ Flt3 ITD/+ mouse Mac1+ cells rep2
Sample type SRA
 
Source name Mac1+ cells
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: Ezh2 fl/fl Runx1 fl/fl Rag1Cre+ Flt3 ITD/+
Extracted molecule total RNA
Extraction protocol 100 cells were FACS-sorted directly into lysis buffer
Picelli et al, Nature Protocols 2014: Full-length RNA-seq from single cells using Smart-seq2.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description 267-DKOITD_S2
Data processing Alignment to mm10 transcriptome with STAR (version 2.4.2a)
Merge Technical replicates with samtools (version 1.1)
Filter non-uniquely mapped reads with samtools (version 1.1)
Count Reads in transcripts with Htseq-count (version 0.6.1p1)
Genome_build: mm10
Supplementary_files_format_and_content: RPKM values
 
Submission date Nov 19, 2015
Last update date May 15, 2019
Contact name Adam Mead
E-mail(s) adam.mead@imm.ox.ac.uk
Organization name University of Oxford
Department Weatherall Institute of Molecular Medicine
Lab HSCB Laboratory
Street address John Radcliffe Hospital
City Oxford
ZIP/Postal code OX3 9DS
Country United Kingdom
 
Platform ID GPL19057
Series (2)
GSE75187 Ezh2 and Runx1 Mutations Targeted to Early Lymphoid Progenitors Collaborate to Promote Early Thymic Progenitor Leukemia [RNA-Seq 2]
GSE75188 Ezh2 and Runx1 Mutations Targeted to Early Lymphoid Progenitors Collaborate to Promote Early Thymic Progenitor Leukemia
Relations
BioSample SAMN04279021
SRA SRX1438376

Supplementary data files not provided
SRA Run SelectorHelp
Processed data are available on Series record
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap