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Sample GSM1944722 Query DataSets for GSM1944722
Status Public on Feb 12, 2018
Title Ezh2 fl/fl Runx1 fl/fl Rag1Cre+ ETPs rep2
Sample type SRA
 
Source name Early Thymic Precursors
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: Ezh2 fl/fl Runx1 fl/fl Rag1Cre+ ETPs
Extracted molecule total RNA
Extraction protocol 100 cells were FACS-sorted directly into lysis buffer
SMARTer; Ramskold et al, Nature Biotechnology 2012: Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cells.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description dblKo2
Data processing Alignment to mm10 genome with transcriptome annotation with Tophat2 (version 2.0.10)
Remove multi-map reads using samtools (version 0.1.19)
Summarise to reads counts with htseq-count (version 0.5.4p5)
Genome_build: mm10
Supplementary_files_format_and_content: RPKM values
 
Submission date Nov 19, 2015
Last update date May 15, 2019
Contact name Adam Mead
E-mail(s) adam.mead@imm.ox.ac.uk
Organization name University of Oxford
Department Weatherall Institute of Molecular Medicine
Lab HSCB Laboratory
Street address John Radcliffe Hospital
City Oxford
ZIP/Postal code OX3 9DS
Country United Kingdom
 
Platform ID GPL17021
Series (2)
GSE75185 Ezh2 and Runx1 Mutations Targeted to Early Lymphoid Progenitors Collaborate to Promote Early Thymic Progenitor Leukemia [RNA-Seq 1]
GSE75188 Ezh2 and Runx1 Mutations Targeted to Early Lymphoid Progenitors Collaborate to Promote Early Thymic Progenitor Leukemia
Relations
BioSample SAMN04279031
SRA SRX1438353

Supplementary data files not provided
SRA Run SelectorHelp
Processed data are available on Series record
Raw data are available in SRA

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