|
| Status |
Public on Jun 15, 2016 |
| Title |
D180_Obese |
| Sample type |
RNA |
| |
|
| Source name |
uterine NK cell
|
| Organism |
Homo sapiens |
| Characteristics |
gestational age in weeks: 8
cell type: uterine NK cell
bmi: BMI = 32.0
crp (ug/ml): hsCRP = 6.6
|
| Treatment protocol |
Uterine decidual mucoa was collected after elective terminations of pregnancy from consenting women at BC Women's Hospital. Tissue natural killer cells were isolated via enzymatic digestion in collagenase and purified by density gradient centrifugation and immuno-magnetic bead purification using a NK cell enrichment cocktail (Stem Cell Technology).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Ambion RNAqueous Total RNA Isolation columns, followed by Dnase I treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with an Agilent Bioanalyzer; only samples with RIN values >7 were used.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Obese_sample5
|
| Data processing |
Raw expression data were log transformed and quantile normalized using he limma library beadarray packages of the Bioconductor open-source project in the R statistical environment
|
| |
|
| Submission date |
Nov 17, 2015 |
| Last update date |
Jun 15, 2016 |
| Contact name |
Alexander G Beristain |
| Phone |
604-875-3573
|
| Organization name |
The University of British Columbia
|
| Department |
Obstetrics & Gynecology
|
| Lab |
Beristain lab
|
| Street address |
950 West 28th Ave
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 4H4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE75091 |
Genome-wide analysis of uterine natural killer cell gene expression from lean and obese women in early pregnancy |
|
