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Status |
Public on May 01, 2016 |
Title |
Fibroblast_S2_c36_sc1 |
Sample type |
genomic |
|
|
Source name |
Primary fibroblast cell line S2, cell 36, sister cell 1
|
Organism |
Homo sapiens |
Characteristics |
gender: Female cell line: S2
|
Treatment protocol |
Single fibroblasts were plated separately and the two cells that derived following a cell division were isolated individually.
|
Growth protocol |
Primary fibroblasts were grown in 75-cm2 plastic flasks (BD Falcon, USA) under standard culture conditions in Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) (Gibco, USA) medium complemented with 10% Fetal Bovine Serum (FBS) (Thermo Scientific, USA).
|
Extracted molecule |
genomic DNA |
Extraction protocol |
The genome of collected single cells was amplified using either Genomiphi V2 (GE Healthcare, UK) or RepliG-sc (Qiagen, NL) whole genome amplification kit.
|
Label |
Cy5,Cy3
|
Label protocol |
The samples were not labelled before the hybridization. The staining is based on post-hybridization Cy5/Cy3 staining by extension, according to the manual of Infinium II Assay (Illumina, USA).
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|
|
Hybridization protocol |
According to the manual of Infinium II Assay (Illumina, USA)
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Scan protocol |
According to the manual of Infinium II Assay (Illumina, USA)
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Description |
Genomiphi V2 whole genome amplified material.
|
Data processing |
Raw data were further processed using GenomeStudio.
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|
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Submission date |
Nov 17, 2015 |
Last update date |
May 01, 2016 |
Contact name |
Eftychia Dimitriadou |
E-mail(s) |
eftychia.dimitriadou@med.kuleuven.be
|
Organization name |
KU Leuven
|
Lab |
Cytogenetics and Genome Research
|
Street address |
Herestraat 49, ON1, bus 602
|
City |
Leuven |
ZIP/Postal code |
3000 |
Country |
Belgium |
|
|
Platform ID |
GPL13829 |
Series (2) |
GSE75088 |
Segmental chromosomal imbalances arise at high frequency in human fibroblasts (S2) |
GSE75116 |
Segmental chromosomal imbalances arise at high frequency in human fibroblasts |
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