|
Status |
Public on May 26, 2009 |
Title |
LowSES 8 |
Sample type |
RNA |
|
|
Source name |
Peripheral blood CD2+ leukocytes
|
Organism |
Homo sapiens |
Characteristics |
other: Low socioeconomic status (SES)
|
Extracted molecule |
total RNA |
Extraction protocol |
Peripheral blood leukocytes were isolated from fresh whole blood by ficol density gradient, CD2+ cells were isolated by MACS immunomagnetic positive selection, and total RNA was extracted using Qiagen RNEasy.
|
Label |
biotin
|
Label protocol |
Standard Affymetrix probe synthesis for gene expression profiling (carried out in UCLA DNA Microarray Core).
|
|
|
Hybridization protocol |
Standard Affymetrix hybridization protocol for gen expression profiling (carried out in UCLA DNA Microarray Core).
|
Scan protocol |
Standard Affymetrixscan protocol for gene expression profiling (carried out in UCLA DNA Microarray Core).
|
Description |
Affymetrix U133A
|
Data processing |
RMA (RMAExpress) processing of Affymetrix .CEL files generated by UCLA DNA Microarray Core.
|
|
|
Submission date |
May 24, 2007 |
Last update date |
Mar 29, 2010 |
Contact name |
Steve Cole |
Organization name |
UCLA School of Medicine
|
Department |
Medicine
|
Street address |
11-934 Factor Bldg, UCLA
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095-1678 |
Country |
USA |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE7894 |
Differential gene expression in peripheral blood CD2+ leukocytes from PATH particpants |
|