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Sample GSM1939649 Query DataSets for GSM1939649
Status Public on Jul 05, 2018
Title Tfh from TONS 2 [exon-level]
Sample type RNA
Source name Ex-vivo Tfh cells
Organism Homo sapiens
Characteristics pathology: Tonsillectomy
treatment: Untreated
Extracted molecule total RNA
Extraction protocol RNA was extracted using AllPrep ARN/ADN kit (Qiagen, Valencia, CA)
Label biotin
Label protocol Biotinylated double strand cDNA targets were prepared with the NuGEN Ovation PicoSL WTA System V2 Kit followed by the NuGEN Encore Biotin Module Kitaccording to manufacturer recommendations
Hybridization protocol Following fragmentation and end-labeling, 4.7 μg of cDNAs were hybridized for 16 hours at 45°C on GeneChip® Human Transcriptome Array 2.0 (Affymetrix)
Scan protocol GeneChips were scanned with the GeneChip® Scanner 3000 7G (Affymetrix) at a resolution of 0.7 µm
Description Gene expression from tonsil CD4 Tfh cells
Data processing Raw data (.CEL Intensity files) were extracted from the scanned images using the Affymetrix GeneChip® Command Console (AGCC) version 4.1.2. CEL files were further processed with Affymetrix Expression Console software version 1.4.1 using Robust Multi-array Average (RMA) algorithms with default settings at the gene and exon levels
Submission date Nov 13, 2015
Last update date Jul 05, 2018
Contact name Patricia Amé-Thomas
Organization name INSERM UMR1236
Department Faculté de Médecine
Street address 2 Avenue du Pr Léon Bernard
City Rennes
ZIP/Postal code 35033
Country France
Platform ID GPL17585
Series (1)
GSE74982 Expression data of T follicular helper (Tfh) and memory CD4 T cells from non malignant tonsils (TONS).
Alternative to GSM1939643 (gene-level analysis)

Data table header descriptions
VALUE RMA signal intensity

Data table
JUC01000001.hg.1 6.510754
JUC01000002.hg.1 4.986691
JUC01000003.hg.1 6.379063
JUC01000004.hg.1 5.191413
JUC01000005.hg.1 7.489969
JUC01000006.hg.1 7.450092
JUC01000007.hg.1 2.732039
JUC01000008.hg.1 5.378406
JUC01000009.hg.1 2.923908
JUC01000010.hg.1 3.062323
JUC01000011.hg.1 10.65969
JUC01000012.hg.1 7.72011
JUC01000013.hg.1 10.68578
JUC01000014.hg.1 8.290683
JUC01000015.hg.1 4.669574
JUC01000016.hg.1 7.027443
JUC01000017.hg.1 6.409217
JUC01000018.hg.1 6.274139
JUC01000019.hg.1 7.475702
JUC01000020.hg.1 7.475702

Total number of rows: 914585

Table truncated, full table size 23382 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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