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Sample GSM1939645 Query DataSets for GSM1939645
Status Public on Jul 05, 2018
Title Memory T CD4 from TONS 1 [exon-level]
Sample type RNA
Source name Ex-vivo memory CD4 T cells
Organism Homo sapiens
Characteristics pathology: Tonsillectomy
treatment: Untreated
Extracted molecule total RNA
Extraction protocol RNA was extracted using AllPrep ARN/ADN kit (Qiagen, Valencia, CA)
Label biotin
Label protocol Biotinylated double strand cDNA targets were prepared with the NuGEN Ovation PicoSL WTA System V2 Kit followed by the NuGEN Encore Biotin Module Kitaccording to manufacturer recommendations
Hybridization protocol Following fragmentation and end-labeling, 4.7 μg of cDNAs were hybridized for 16 hours at 45°C on GeneChip® Human Transcriptome Array 2.0 (Affymetrix)
Scan protocol GeneChips were scanned with the GeneChip® Scanner 3000 7G (Affymetrix) at a resolution of 0.7 µm
Description Gene expression from tonsil naive CD4 T cells
Data processing Raw data (.CEL Intensity files) were extracted from the scanned images using the Affymetrix GeneChip® Command Console (AGCC) version 4.1.2. CEL files were further processed with Affymetrix Expression Console software version 1.4.1 using Robust Multi-array Average (RMA) algorithms with default settings at the gene and exon levels
Submission date Nov 13, 2015
Last update date Jul 05, 2018
Contact name Patricia Amé-Thomas
Organization name INSERM UMR1236
Department Faculté de Médecine
Street address 2 Avenue du Pr Léon Bernard
City Rennes
ZIP/Postal code 35033
Country France
Platform ID GPL17585
Series (1)
GSE74982 Expression data of T follicular helper (Tfh) and memory CD4 T cells from non malignant tonsils (TONS).
Alternative to GSM1939639 (gene-level analysis)

Data table header descriptions
VALUE RMA signal intensity

Data table
JUC01000001.hg.1 6.62389
JUC01000002.hg.1 4.701872
JUC01000003.hg.1 5.427561
JUC01000004.hg.1 4.598932
JUC01000005.hg.1 6.68515
JUC01000006.hg.1 7.634854
JUC01000007.hg.1 3.115432
JUC01000008.hg.1 6.413307
JUC01000009.hg.1 2.336666
JUC01000010.hg.1 3.118695
JUC01000011.hg.1 10.61082
JUC01000012.hg.1 7.026506
JUC01000013.hg.1 10.8919
JUC01000014.hg.1 8.661064
JUC01000015.hg.1 4.959645
JUC01000016.hg.1 6.798861
JUC01000017.hg.1 6.961573
JUC01000018.hg.1 6.6125
JUC01000019.hg.1 7.344717
JUC01000020.hg.1 7.344717

Total number of rows: 914585

Table truncated, full table size 23383 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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