|
| Status |
Public on Feb 22, 2016 |
| Title |
K562 shKDM2B_1 |
| Sample type |
RNA |
| |
|
| Source name |
K562_shKDM2B
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: K562
|
| Treatment protocol |
Cells were infected with shKDM2B and RNA harvested after 6 days
|
| Growth protocol |
Human leukemia cell lines were maintained in IMDM supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Hybridization was performed using a GeneChip Hybridization Oven 640 overnight at 45°C. Microarray washing and staining was done using a GeneChip Fluidics Station 450
|
| Scan protocol |
GeneChip Scanner 3000 7G (Affymetrix)
|
| Data processing |
Probe level analysis including background subtraction and quantile normalization took placed with the Robust Multi Array Average Algorithm (RMA) using the Affymetrix Expression Console Software 1.3
|
| |
|
| Submission date |
Nov 12, 2015 |
| Last update date |
Feb 22, 2016 |
| Contact name |
Alexandros Tzatsos |
| E-mail(s) |
atzatsos@gwu.edu
|
| Phone |
2029949104
|
| Organization name |
George Washington University
|
| Department |
GW Cancer Center
|
| Lab |
Cancer Epigenetics Laboratory
|
| Street address |
800 22nd St NW SEH 8850
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20052 |
| Country |
USA |
| |
|
| Platform ID |
GPL15207 |
| Series (2) |
| GSE70729 |
Role of the histone demethylase KDM2B in hematopoietic homeostasis and malignancies |
| GSE74959 |
Role of the histone demethylase KDM2B in hematopoietic homeostasis and malignancies [human cell line gene expression] |
|
