NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1939034 Query DataSets for GSM1939034
Status Public on Feb 22, 2016
Title DND41 shControl_2
Sample type RNA
 
Source name DND41_shControl
Organism Homo sapiens
Characteristics cell line: DND41
Treatment protocol Cells were infected with shKDM2B and RNA harvested after 6 days
Growth protocol Human leukemia cell lines were maintained in IMDM supplemented with 10% FBS
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Hybridization was performed using a GeneChip Hybridization Oven 640 overnight at 45°C. Microarray washing and staining was done using a GeneChip Fluidics Station 450
Scan protocol GeneChip Scanner 3000 7G (Affymetrix)
Data processing Probe level analysis including background subtraction and quantile normalization took placed with the Robust Multi Array Average Algorithm (RMA) using the Affymetrix Expression Console Software 1.3
 
Submission date Nov 12, 2015
Last update date Feb 22, 2016
Contact name Alexandros Tzatsos
E-mail(s) atzatsos@gwu.edu
Phone 2029949104
Organization name George Washington University
Department GW Cancer Center
Lab Cancer Epigenetics Laboratory
Street address 800 22nd St NW SEH 8850
City Washington
State/province DC
ZIP/Postal code 20052
Country USA
 
Platform ID GPL15207
Series (2)
GSE70729 Role of the histone demethylase KDM2B in hematopoietic homeostasis and malignancies
GSE74959 Role of the histone demethylase KDM2B in hematopoietic homeostasis and malignancies [human cell line gene expression]

Data table header descriptions
ID_REF
VALUE RMA Signal

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.38235
AFFX-BioB-M_at 8.34939
AFFX-BioB-3_at 8.07312
AFFX-BioC-5_at 9.66292
AFFX-BioC-3_at 10.0201
AFFX-BioDn-5_at 10.9915
AFFX-BioDn-3_at 12.2074
AFFX-CreX-5_at 13.304
AFFX-CreX-3_at 13.6374
AFFX-DapX-5_at 6.83643
AFFX-DapX-M_at 7.76671
AFFX-DapX-3_at 8.74708
AFFX-LysX-5_at 4.1467
AFFX-LysX-M_at 5.04217
AFFX-LysX-3_at 5.88231
AFFX-PheX-5_at 5.04997
AFFX-PheX-M_at 5.31053
AFFX-PheX-3_at 6.34891
AFFX-ThrX-5_at 5.1675
AFFX-ThrX-M_at 5.56438

Total number of rows: 49395

Table truncated, full table size 1033 Kbytes.




Supplementary file Size Download File type/resource
GSM1939034_DND41_shControl_2.CEL.gz 2.1 Mb (ftp)(http) CEL
GSM1939034_DND41_shControl_2.rma.chp.gz 346.7 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap