|
Status |
Public on Feb 22, 2016 |
Title |
DND41 shControl_1 |
Sample type |
RNA |
|
|
Source name |
DND41_shControl
|
Organism |
Homo sapiens |
Characteristics |
cell line: DND41
|
Treatment protocol |
Cells were infected with shKDM2B and RNA harvested after 6 days
|
Growth protocol |
Human leukemia cell lines were maintained in IMDM supplemented with 10% FBS
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy kit
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
|
|
Hybridization protocol |
Hybridization was performed using a GeneChip Hybridization Oven 640 overnight at 45°C. Microarray washing and staining was done using a GeneChip Fluidics Station 450
|
Scan protocol |
GeneChip Scanner 3000 7G (Affymetrix)
|
Data processing |
Probe level analysis including background subtraction and quantile normalization took placed with the Robust Multi Array Average Algorithm (RMA) using the Affymetrix Expression Console Software 1.3
|
|
|
Submission date |
Nov 12, 2015 |
Last update date |
Feb 22, 2016 |
Contact name |
Alexandros Tzatsos |
E-mail(s) |
atzatsos@gwu.edu
|
Phone |
2029949104
|
Organization name |
George Washington University
|
Department |
GW Cancer Center
|
Lab |
Cancer Epigenetics Laboratory
|
Street address |
800 22nd St NW SEH 8850
|
City |
Washington |
State/province |
DC |
ZIP/Postal code |
20052 |
Country |
USA |
|
|
Platform ID |
GPL15207 |
Series (2) |
GSE70729 |
Role of the histone demethylase KDM2B in hematopoietic homeostasis and malignancies |
GSE74959 |
Role of the histone demethylase KDM2B in hematopoietic homeostasis and malignancies [human cell line gene expression] |
|