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Sample GSM1939032 Query DataSets for GSM1939032
Status Public on Feb 22, 2016
Title JURKAT shKDM2B_2
Sample type RNA
Source name JURKAT_shKDM2B
Organism Homo sapiens
Characteristics cell line: JURKAT
Treatment protocol Cells were infected with shKDM2B and RNA harvested after 6 days
Growth protocol Human leukemia cell lines were maintained in IMDM supplemented with 10% FBS
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
Hybridization protocol Hybridization was performed using a GeneChip Hybridization Oven 640 overnight at 45°C. Microarray washing and staining was done using a GeneChip Fluidics Station 450
Scan protocol GeneChip Scanner 3000 7G (Affymetrix)
Data processing Probe level analysis including background subtraction and quantile normalization took placed with the Robust Multi Array Average Algorithm (RMA) using the Affymetrix Expression Console Software 1.3
Submission date Nov 12, 2015
Last update date Feb 22, 2016
Contact name Alexandros Tzatsos
Phone 2029949104
Organization name George Washington University
Department GW Cancer Center
Lab Cancer Epigenetics Laboratory
Street address 800 22nd St NW SEH 8850
City Washington
State/province DC
ZIP/Postal code 20052
Country USA
Platform ID GPL15207
Series (2)
GSE70729 Role of the histone demethylase KDM2B in hematopoietic homeostasis and malignancies
GSE74959 Role of the histone demethylase KDM2B in hematopoietic homeostasis and malignancies [human cell line gene expression]

Data table header descriptions

Data table
AFFX-BioB-5_at 7.9193
AFFX-BioB-M_at 8.41829
AFFX-BioB-3_at 7.80465
AFFX-BioC-5_at 9.48843
AFFX-BioC-3_at 10.0511
AFFX-BioDn-5_at 10.9949
AFFX-BioDn-3_at 12.1308
AFFX-CreX-5_at 13.4023
AFFX-CreX-3_at 13.9299
AFFX-DapX-5_at 10.0705
AFFX-DapX-M_at 10.9517
AFFX-DapX-3_at 11.9247
AFFX-LysX-5_at 6.94901
AFFX-LysX-M_at 7.71914
AFFX-LysX-3_at 8.2796
AFFX-PheX-5_at 8.08268
AFFX-PheX-M_at 8.39182
AFFX-PheX-3_at 8.41095
AFFX-ThrX-5_at 7.93751
AFFX-ThrX-M_at 8.80769

Total number of rows: 49395

Table truncated, full table size 1033 Kbytes.

Supplementary file Size Download File type/resource
GSM1939032_Jurkat_shKDM2B_2.CEL.gz 1.9 Mb (ftp)(http) CEL
GSM1939032_Jurkat_shKDM2B_2.rma.chp.gz 345.6 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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