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Sample GSM1938986 Query DataSets for GSM1938986
Status Public on Mar 16, 2016
Title HuES64
Sample type SRA
 
Source name Diploid ES cell line
Organism Homo sapiens
Characteristics cell line: HuES64
passage: <20
differentiation status: Undifferentiated
Extracted molecule polyA RNA
Extraction protocol RNA was isolated using the Total RNA Purification Micro Kit (Norgen). For RNA sequencing analysis, poly(A) pull-down was used to enrich mRNAs from total RNA samples (200 ng–1 µg per sample, RIN > 8)
Libraries were prepared using the Illumina TruSeq RNA prep kit
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Data processing Reads were mapped to the human reference genome (NCBI/build37.2) using Tophat (version 2.0.4)
Fragments per kilobase of transcript per million mapped reads (FPKM) values were calculated using Cufflinks (version 2.0.2)
Genome_build: NCBI/build 37.2
Supplementary_files_format_and_content: Tab-delimited text file includes FPKM values for each sample
 
Submission date Nov 12, 2015
Last update date May 15, 2019
Contact name Ido Sagi
Organization name The Hebrew University of Jerusalem
Department Genetics
Lab Nissim Benvenisty Lab, The Azrieli Center for Stem Cells and Genetic Research
Street address Givat Ram
City Jerusalem
ZIP/Postal code 91904
Country Israel
 
Platform ID GPL16791
Series (2)
GSE71458 Derivation and differentiation of haploid human embryonic stem cells
GSE74953 Derivation and differentiation of haploid human embryonic stem cells [RNA-Seq 2]
Relations
BioSample SAMN04262533
SRA SRX1429049

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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