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Sample GSM1938095 Query DataSets for GSM1938095
Status Public on Nov 13, 2015
Title HCT116 cells transfected with a ZNF582-AS1 expression vector 2
Sample type RNA
 
Source name HCT116
Organism Homo sapiens
Characteristics cell line: HCT116
vector: ZNF582-AS1
Treatment protocol HCT116 cells were transfected with a ZNF582-AS1 expression vector or an empty vector. Seventy-two hours after transfection, total RNA was extracted
Growth protocol HCT116 cells were cultured in McCoy's 5A Medium supplemented with 10% heat-inactivated fetal bovine serum. The cultures were incubated at 37 ÂșC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using a RNeasy Mini Kit (QIAGEN).
Label Cy3
Label protocol Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
 
Hybridization protocol Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
Scan protocol Array was scanned with an Agilent G2565BA Microarray Scanner.
Description gene expression in HCT116 cells transfected with a ZNF582-AS1 expression vector (replicate 2)
Data processing The microarray data were analyzed using GeneSpring GX version 13
 
Submission date Nov 12, 2015
Last update date Apr 23, 2018
Contact name Hiromu Suzuki
E-mail(s) hsuzuki@sapmed.ac.jp
Phone +81-11-611-2111
Organization name Sapporo Medical University
Department Department of Molecular Biology
Street address S1, W17, Chuo-ku
City Sapporo
State/province Hokkaido
ZIP/Postal code 060-8556
Country Japan
 
Platform ID GPL16699
Series (1)
GSE74945 A genomic screen for long noncoding RNA genes epigenetically silenced by aberrant DNA methylation in colorectal cancer
Relations
Reanalyzed by GSE113533

Data table header descriptions
ID_REF
VALUE gProcessed Signal

Data table
ID_REF VALUE
1 4.28E+04
2 3.62E+00
3 3.65E+00
4 3.98E+03
5 6.74E+00
6 3.70E+00
7 3.72E+00
8 1.67E+01
9 8.75E+04
10 3.11E+02
11 2.00E+02
12 1.45E+03
13 7.60E+01
14 3.79E+00
15 8.12E+00
16 3.80E+00
17 5.35E+01
18 1.51E+02
19 1.25E+02
20 3.46E+03

Total number of rows: 62976

Table truncated, full table size 911 Kbytes.




Supplementary file Size Download File type/resource
GSM1938095_ZNF582_AS1_2.txt.gz 12.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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