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Sample GSM1937086 Query DataSets for GSM1937086
Status Public on May 16, 2016
Title Combined CUL4A siRNA with IL-6 stimulation treated group 2
Sample type RNA
Source name colon adenocarcinoma
Organism Homo sapiens
Characteristics cell line: HCT116
agent: IL-6+ siCUL4A
Treatment protocol Male BALB/c athymic nude mice (4–5 weeks old) were injected in right armpit subcutaneously with 1×107 of HCT116 cells, in a volume of 2ml of PBS, Once the tumor size reached approximately 5 mm in diameter, the mice were randomly divided into groups: 1) PBS control group with tumors untreated; 2) IL-6 stimulation treated group, 3) Combined CUL4A siRNA with IL-6 stimulation treated group.30 ul siRNA/oligofectamine mixture containing 400 pmol siRNA or/and 600pg IL-6 every four days. After a month, the mice were sacrificed and the tumor tissues were taken.
Growth protocol The human colon adenocarcinoma cell lines HCT116 were cultured inDulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 100 units/m of penicillin and streptomycin. Cultures were incubated at 37°C in a humidified incubator at 5% CO2.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45℃ on Affymetrix® Multispecies miRNA 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip® Scanner 3000.
Description Expression data for xenograft tumor treated with IL-6 combined CUL4A siRNA
Data processing Affymetrix GeneChip Command Console (version 4.0, Affymetrix) was used to analyze array images to get raw data. Raw data were normalized with the RMA algorithm by Gene Spring Software 13.0 (Agilent)
Submission date Nov 10, 2015
Last update date May 16, 2016
Contact name Weiguo Ren
Phone 13054196004
Organization name hird Xiangya Hospital of Central South University
Street address No.216, Tongzipo, Yuelu District
City Changsha
State/province Hunan
ZIP/Postal code 410013
Country China
Platform ID GPL19117
Series (1)
GSE74891 Expression data for xenograft tumor treated with IL-6 or siCUL4A.

Data table header descriptions
VALUE RMA signal intensity

Data table
MIMAT0000001_st 12.05936
MIMAT0015091_st 0.9933159
MIMAT0000002_st 1.057903
MIMAT0015092_st 1.08265
MIMAT0020301_st 0.8485692
MIMAT0000003_st 1.124761
MIMAT0020302_st 1.173884
MIMAT0000004_st 0.9139317
MIMAT0000005_st 1.169446
MIMAT0015093_st 1.295326
MIMAT0020303_st 1.169037
MIMAT0000006_st 0.9357284
MIMAT0020304_st 0.9890755
MIMAT0000007_st 0.7794019
MIMAT0015094_st 1.346236
MIMAT0000008_st 1.018969
MIMAT0020305_st 1.045291
MIMAT0000009_st 0.8315657
MIMAT0020306_st 0.9193653
MIMAT0000010_st 0.8633664

Total number of rows: 36249

Table truncated, full table size 880 Kbytes.

Supplementary file Size Download File type/resource
GSM1937086_Tumor_IL-6+_siCUL4A-2.CEL.gz 756.1 Kb (ftp)(http) CEL
Processed data included within Sample table

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