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Sample GSM1937085 Query DataSets for GSM1937085
Status Public on May 16, 2016
Title Combined CUL4A siRNA with IL-6 stimulation treated group 1
Sample type RNA
Source name colon adenocarcinoma
Organism Homo sapiens
Characteristics cell line: HCT116
agent: IL-6+ siCUL4A
Treatment protocol Male BALB/c athymic nude mice (4–5 weeks old) were injected in right armpit subcutaneously with 1×107 of HCT116 cells, in a volume of 2ml of PBS, Once the tumor size reached approximately 5 mm in diameter, the mice were randomly divided into groups: 1) PBS control group with tumors untreated; 2) IL-6 stimulation treated group, 3) Combined CUL4A siRNA with IL-6 stimulation treated group.30 ul siRNA/oligofectamine mixture containing 400 pmol siRNA or/and 600pg IL-6 every four days. After a month, the mice were sacrificed and the tumor tissues were taken.
Growth protocol The human colon adenocarcinoma cell lines HCT116 were cultured inDulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 100 units/m of penicillin and streptomycin. Cultures were incubated at 37°C in a humidified incubator at 5% CO2.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45℃ on Affymetrix® Multispecies miRNA 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip® Scanner 3000.
Description Expression data for xenograft tumor treated with IL-6 combined CUL4A siRNA
Data processing Affymetrix GeneChip Command Console (version 4.0, Affymetrix) was used to analyze array images to get raw data. Raw data were normalized with the RMA algorithm by Gene Spring Software 13.0 (Agilent)
Submission date Nov 10, 2015
Last update date May 16, 2016
Contact name Weiguo Ren
Phone 13054196004
Organization name hird Xiangya Hospital of Central South University
Street address No.216, Tongzipo, Yuelu District
City Changsha
State/province Hunan
ZIP/Postal code 410013
Country China
Platform ID GPL19117
Series (1)
GSE74891 Expression data for xenograft tumor treated with IL-6 or siCUL4A.

Data table header descriptions
VALUE RMA signal intensity

Data table
MIMAT0000001_st 12.62197
MIMAT0015091_st 1.051047
MIMAT0000002_st 1.046937
MIMAT0015092_st 1.022664
MIMAT0020301_st 1.075207
MIMAT0000003_st 1.006751
MIMAT0020302_st 1.287792
MIMAT0000004_st 1.263729
MIMAT0000005_st 1.309277
MIMAT0015093_st 1.326111
MIMAT0020303_st 1.220614
MIMAT0000006_st 1.054247
MIMAT0020304_st 1.056951
MIMAT0000007_st 1.083308
MIMAT0015094_st 0.9833745
MIMAT0000008_st 1.227963
MIMAT0020305_st 1.217346
MIMAT0000009_st 0.9770964
MIMAT0020306_st 1.063355
MIMAT0000010_st 1.116692

Total number of rows: 36249

Table truncated, full table size 881 Kbytes.

Supplementary file Size Download File type/resource
GSM1937085_Tumor_IL-6+_siCUL4A-1.CEL.gz 739.0 Kb (ftp)(http) CEL
Processed data included within Sample table

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