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Sample GSM1936094 Query DataSets for GSM1936094
Status Public on Feb 01, 2016
Title ChIP-seq - mES - H3K36me3 - 12hr KD, Control ASO rep2
Sample type SRA
Source name Embryonic Stem Cells
Organism Mus musculus
Characteristics cell type: ES cells
cell line: v6.5
gender: male
antibody: Abcam, Cat #ab9050
Treatment protocol All cells were fixed with 1% formaldehyde for 10 minutes at room temperature.
Growth protocol mES cells were grown on gelatin plates with serum and LIF.
Extracted molecule genomic DNA
Extraction protocol Lysates were produced from soncated cell material and transcription factor associated DNA was enriched with specific antibodies.
Libraries were prepared according to NEBNext kit.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NextSeq 500
Data processing After demultiplexing, reads were stripped adaptors and mapped to the mouse (mm9) genome using Bowtie2 and visualized as bigWig files. Peaks were called using MACS2.
Genome_build: mm9
Supplementary_files_format_and_content: bigWig files of ChIP-seq read density
Submission date Nov 09, 2015
Last update date May 15, 2019
Contact name Ryan Alexander Flynn
Organization name Stanford University
Street address 380 Roth Way, Room 265
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platform ID GPL19057
Series (2)
GSE69140 7SK-BAF axis controls pervasive transcription at enhancers [ChIP-Seq]
GSE69143 7SK-BAF axis controls pervasive transcription at enhancers
BioSample SAMN04252811
SRA SRX1425197

Supplementary file Size Download File type/resource 273.5 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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