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Sample GSM1923352 Query DataSets for GSM1923352
Status Public on Nov 03, 2015
Title cultured PBMC from healthy control stimulated with PHA
Sample type RNA
Source name cultured healthy control PBMC with PHA
Organism Homo sapiens
Characteristics tissue: PBMC
disease state: healthy control
gender: female
age: 29
Treatment protocol To induce PBMC activation, Phytohemagglutinin (PHA) (ThermoFisher) (0.5%) was used.
Growth protocol PBMCs were isolated from whole blood and cryopreserved in liquid nitrogen until used. After thawing, PBMCs were cultured in a P48 plate (200.000cells/well) for 72h.
Extracted molecule total RNA
Extraction protocol miRNeasy mini kit (Qiagen)
Label biotin
Label protocol Total RNA was labeled using FlashTag Biotin HSR RNA labeling kit (Genisphere)
Hybridization protocol GeneChip Hybridization, Wash and Stain kit was used for hybridization in a GeneChip Hybridization oven 640 and in a GeneChip Fluidics Station 450 using the protocol FS450-0002, as explained in the protocol provided by Affymetrix.
Scan protocol Arrays were scanned in a GeneChip 7G scanner and analysed in Affymetrix GeneChip Command Console Scan Control v4.0.0.1567.
Description 08-650CNTRL_PHA
Data processing Quality control, normalization and summarization of the data were performed in Expression Console software build (Affymetrix). Only human probes.
Submission date Nov 02, 2015
Last update date Nov 03, 2015
Contact name Maider Muñoz-Culla
Organization name Biodonostia Institute
Street address Paseo Dr.Begiristain s/n
City San Sebastian
ZIP/Postal code 20014
Country Spain
Platform ID GPL19117
Series (2)
GSE74582 Small nucleolar RNA and microRNA expression data from an MS patient's and a healthy control's PBMC cultures stimulated with phytohemagglutinin.
GSE74586 SncRNA (microRNA & snoRNA) expression in leucocytes from multiple sclerosis relapse and remission and cultured peripheral blood mononuclear cells

Data table header descriptions
VALUE log2 RMA-DABG signal
Detection p-value

Data table
ID_REF VALUE Detection Detection p-value
MIMAT0000062_st 12.00239 P 8.72E-16
MIMAT0004481_st 0.6273047 A 0.6242406
MIMAT0010195_st 0.5987374 A 0.696739
MIMAT0000063_st 11.49014 P 8.72E-16
MIMAT0004482_st 0.6167038 A 0.611809
MIMAT0000064_st 10.32149 P 8.72E-16
MIMAT0026472_st 1.17383 A 0.196831
MIMAT0000065_st 12.11065 P 8.72E-16
MIMAT0004484_st 5.328849 P 5.63E-06
MIMAT0000066_st 9.668295 P 8.72E-16
MIMAT0004485_st 1.481916 A 0.2545503
MIMAT0000067_st 9.62558 P 9.48E-16
MIMAT0004486_st 0.9239771 P 0.04769592
MIMAT0004487_st 0.6783194 A 0.9588795
MIMAT0000068_st 9.076405 P 8.72E-16
MIMAT0004488_st 0.7525579 A 0.3487098
MIMAT0000069_st 13.08696 P 8.72E-16
MIMAT0004489_st 2.452412 P 0.01924715
MIMAT0000070_st 13.70132 P 8.72E-16
MIMAT0000071_st 8.770983 P 8.72E-16

Total number of rows: 6658

Table truncated, full table size 241 Kbytes.

Supplementary file Size Download File type/resource
GSM1923352_08-650CNTRL_PHA_miRNA-4_0_.CEL.gz 750.7 Kb (ftp)(http) CEL
Processed data included within Sample table

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