|
Status |
Public on Nov 03, 2015 |
Title |
cultured PBMC from MS patient stimulated with PHA |
Sample type |
RNA |
|
|
Source name |
cultured MS PBMC with PHA
|
Organism |
Homo sapiens |
Characteristics |
tissue: PBMC disease state: MS patient gender: female age: 40
|
Treatment protocol |
To induce PBMC activation, Phytohemagglutinin (PHA) (ThermoFisher) (0.5%) was used.
|
Growth protocol |
PBMCs were isolated from whole blood and cryopreserved in liquid nitrogen until used. After thawing, PBMCs were cultured in a P48 plate (200.000cells/well) for 72h.
|
Extracted molecule |
total RNA |
Extraction protocol |
miRNeasy mini kit (Qiagen)
|
Label |
biotin
|
Label protocol |
Total RNA was labeled using FlashTag Biotin HSR RNA labeling kit (Genisphere)
|
|
|
Hybridization protocol |
GeneChip Hybridization, Wash and Stain kit was used for hybridization in a GeneChip Hybridization oven 640 and in a GeneChip Fluidics Station 450 using the protocol FS450-0002, as explained in the protocol provided by Affymetrix.
|
Scan protocol |
Arrays were scanned in a GeneChip 7G scanner and analysed in Affymetrix GeneChip Command Console Scan Control v4.0.0.1567.
|
Description |
03-029EM_PHA
|
Data processing |
Quality control, normalization and summarization of the data were performed in Expression Console software build 1.4.0.38 (Affymetrix). Only human probes.
|
|
|
Submission date |
Nov 02, 2015 |
Last update date |
Nov 03, 2015 |
Contact name |
Maider Muñoz-Culla |
E-mail(s) |
maider.munoz@ehu.eus
|
Organization name |
Biodonostia Institute
|
Street address |
Paseo Dr.Begiristain s/n
|
City |
San Sebastian |
ZIP/Postal code |
20014 |
Country |
Spain |
|
|
Platform ID |
GPL19117 |
Series (2) |
GSE74582 |
Small nucleolar RNA and microRNA expression data from an MS patient's and a healthy control's PBMC cultures stimulated with phytohemagglutinin. |
GSE74586 |
SncRNA (microRNA & snoRNA) expression in leucocytes from multiple sclerosis relapse and remission and cultured peripheral blood mononuclear cells |
|