|Public on Feb 01, 2016
|cancer type: Non-small cell lung cancer (NSCLC)
perfusion group: low
|Human tumor samples were hormogenized in Trizol and the total RNA was extracted using RNeasy Mini Kit (Qiagen) following the manufacturer's protocol.
1-3 ug of total RNA was processed for library generation using Ovation human FFPE RNA-Seq library systems (NuGen) following the manufacturer’s protocol.
|Illumina NextSeq 500
|Sequencing reads were aligned to human genome assembly hg19 by TopHat.
Transcripts were assembled and the abundance was estimated by Cufflinks.
The number of reads aligned to RefSeq annotated genes' exons were counted and further normalized to be of unit of FPKM ( number of fragments per kilo base of exon per million reads mapped) by Cuffnorm.
Supplementary_files_format_and_content: text file of gene read count values
Supplementary_files_format_and_content: text file of gene FPKM values
|Oct 29, 2015
|Last update date
|May 15, 2019
|UT Southwestern Medical Center
|Children's Research Institute
|6000 Harry Hines Blvd
|Gene expression profiling in metabolically heterogeneous human lung tumors