GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1921205 Query DataSets for GSM1921205
Status Public on May 04, 2016
Title S2_Pre_normoxic bedrest (NBR)
Sample type RNA
Source name Human skeletal muscle, Vastus lateralis
Organism Homo sapiens
Characteristics tissue: Vastus lateralis
gender: Male
condition: normoxic bedrest (NBR)
subject: 2
intervention_number: Second
time: Pre
Extracted molecule total RNA
Extraction protocol Biopsies were obtained from the vastus lateralis muscle using the Bergström needle technique. The biopsies were immediately stored in liquid nitrogen until processing. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix miRNA_4.1 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Not provided
Description Subject 2, Second Intervention: normoxic bedrest (NBR), Pre
Data processing The probe-set level intensities of the arrays were normalized using the Robust Multi-array Analysis method (RMA) implemented withinAffymetrix Expression Console version 1.4.1.
Submission date Oct 29, 2015
Last update date May 04, 2016
Contact name Eric Rullman
Phone +46858586777
Organization name Karolinska Institutet
Department Laboratory Medicine
Lab Clinical Physiology
Street address C1:82 Karolinska University Hospital, Huddinge
City Stockholm
ZIP/Postal code 14186
Country Sweden
Platform ID GPL19117
Series (1)
GSE74469 Effects of bedrest and hypoxia on human skeletal muscle miRNA expression

Data table header descriptions
VALUE Robust Multi-array Average (RMA)

Data table
MIMAT0000062_st 12.94
MIMAT0004481_st 0.55
MIMAT0010195_st 0.9
MIMAT0000063_st 13.7
MIMAT0004482_st 4.35
MIMAT0000064_st 12.85
MIMAT0026472_st 1
MIMAT0000065_st 12.79
MIMAT0004484_st 5.33
MIMAT0000066_st 10.7
MIMAT0004485_st 0.97
MIMAT0000067_st 8.97
MIMAT0004486_st 5.26
MIMAT0004487_st 0.52
MIMAT0000068_st 8.06
MIMAT0004488_st 1.46
MIMAT0000069_st 12.68
MIMAT0004489_st 0.86
MIMAT0000070_st 11.22
MIMAT0000071_st 6.01

Total number of rows: 6658

Table truncated, full table size 134 Kbytes.

Supplementary file Size Download File type/resource
GSM1921205_HF_09_S2_NBR_1_F05.CEL.gz 885.3 Kb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap