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Sample GSM1921196 Query DataSets for GSM1921196
Status Public on May 04, 2016
Title S1_Post_hypoxic ambulatory confinement (HAMB)
Sample type RNA
 
Source name Human skeletal muscle, Vastus lateralis
Organism Homo sapiens
Characteristics tissue: Vastus lateralis
gender: Male
condition: hypoxic ambulatory confinement (HAMB)
subject: 1
intervention_number: First
time: Post
Extracted molecule total RNA
Extraction protocol Biopsies were obtained from the vastus lateralis muscle using the Bergström needle technique. The biopsies were immediately stored in liquid nitrogen until processing. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix miRNA_4.1 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Not provided
Description Subject 1, First Intervention: hypoxic ambulatory confinement (HAMB), Post
Data processing The probe-set level intensities of the arrays were normalized using the Robust Multi-array Analysis method (RMA) implemented withinAffymetrix Expression Console version 1.4.1.
 
Submission date Oct 29, 2015
Last update date May 04, 2016
Contact name Eric Rullman
E-mail(s) eric.rullman@gmail.com
Phone +46858586777
Organization name Karolinska Institutet
Department Laboratory Medicine
Lab Clinical Physiology
Street address C1:82 Karolinska University Hospital, Huddinge
City Stockholm
ZIP/Postal code 14186
Country Sweden
 
Platform ID GPL19117
Series (1)
GSE74469 Effects of bedrest and hypoxia on human skeletal muscle miRNA expression

Data table header descriptions
ID_REF
VALUE Robust Multi-array Average (RMA)

Data table
ID_REF VALUE
MIMAT0000062_st 12.71
MIMAT0004481_st 0.39
MIMAT0010195_st 0.98
MIMAT0000063_st 13.49
MIMAT0004482_st 2.03
MIMAT0000064_st 12.68
MIMAT0026472_st 0.61
MIMAT0000065_st 12.48
MIMAT0004484_st 6.24
MIMAT0000066_st 10.43
MIMAT0004485_st 1.12
MIMAT0000067_st 9.56
MIMAT0004486_st 1.3
MIMAT0004487_st 0.65
MIMAT0000068_st 8.03
MIMAT0004488_st 0.77
MIMAT0000069_st 12.37
MIMAT0004489_st 0.75
MIMAT0000070_st 10.69
MIMAT0000071_st 4.81

Total number of rows: 6658

Table truncated, full table size 134 Kbytes.




Supplementary file Size Download File type/resource
GSM1921196_HF_02_S1_HAMB_2_C09.CEL.gz 893.1 Kb (ftp)(http) CEL
Processed data included within Sample table

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