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Sample GSM1915213 Query DataSets for GSM1915213
Status Public on Aug 22, 2017
Title ISX n3
Sample type RNA
Source name Long-term cultured Human Pancreatic Islet cells
Organism Homo sapiens
Characteristics tissue: Pancreatic Islet cells, long-term culture
Treatment protocol Long-term cultured human pancreatic islet cells were treated with ISX (20uM) or DMSO (0.1%) for 7d with drug/media replacement at day 4.
Growth protocol Human pacreatic islets were cultured for 8-14 months in RPMI-1640 with 10% FBS and Pen/Strep prior to treatment.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the PureLink RNA Mini Kit (Life Technologies) per manufacturers instructions. On-column DNAse treatment was performed during the purification to eliminate trace genomic DNA.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT Plus cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
Hybridization protocol Samples were hybridized with GeneChip Human HTA Arrays (Affymetrix)
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description CHP file generated for Alternative Splicing Analysis from the Expression Console software and then analyzed in the Transcriptome Analysis Console v2.0 for log2 expression values.
Data processing Raw data were processed with the Expression Console (Affymetrix) for RMA summarization and Quantile normalization.
Submission date Oct 21, 2015
Last update date Aug 22, 2017
Contact name Michael A Kalwat
Organization name Indiana Biosciences Research Institute
Department Lilly Diabetes Center of Excellence
Lab Kalwat Lab
Street address 1210 Waterway Blvd Suite 2000
City Indianapolis
State/province IN
ZIP/Postal code 46077
Country USA
Platform ID GPL17585
Series (1)
GSE74240 Effects of isoxazole (ISX) on long-term cultured human islets

Data table header descriptions
VALUE Quantification

Data table
JUC01000001.hg.1 6.96778 0.0330728
JUC01000002.hg.1 3.35462 0.593714
JUC01000003.hg.1 6.62894 0.0052605
JUC01000004.hg.1 6.16001 0.0340324
JUC01000005.hg.1 8.04004 0.00382008
JUC01000006.hg.1 7.13218 0.0314179
JUC01000007.hg.1 5.18431 0.280714
JUC01000008.hg.1 7.1571 0.000127026
JUC01000009.hg.1 3.7665 0.0338628
JUC01000010.hg.1 3.56707 0.00777273
JUC01000011.hg.1 12.0298 1.75899e-06
JUC01000012.hg.1 7.92587 0.0656996
JUC01000013.hg.1 11.5471 1.22474e-07
JUC01000014.hg.1 9.54937 1.54832e-08
JUC01000015.hg.1 5.27731 0.0296454
JUC01000016.hg.1 9.01906 9.10078e-05
JUC01000017.hg.1 7.27242 1.24742e-06
JUC01000018.hg.1 7.90698 0.0696985
JUC01000019.hg.1 8.74322 0.000808949
JUC01000020.hg.1 8.74322 0.000808949

Total number of rows: 914585

Table truncated, full table size 32063 Kbytes.

Supplementary file Size Download File type/resource
GSM1915213_012098_LSH1_4.rma-alt-splice-dabg.chp.gz 9.3 Mb (ftp)(http) CHP
GSM1915213_LSH1_rpt3.CEL.gz 25.4 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data provided as supplementary file

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