|
Status |
Public on Dec 21, 2015 |
Title |
2b |
Sample type |
RNA |
|
|
Source name |
Primary human osteoblasts
|
Organism |
Homo sapiens |
Characteristics |
cy3 data file: 1_Exiqon_19708489_S01.txt cy5 data file: 0_Exiqon_19708489_S01.txt tissue: Primary bone cells condition: Osteoarthritis
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using High Pure RNA Isolation kit (Roche Diagnostics, Indianapolis, USA)
|
Label |
biotin
|
Label protocol |
750 ng total RNA from both sample and reference was labeled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy3™/Hy5™ (Exiqon, Denmark) following the procedure described by the manufacturer
|
|
|
Hybridization protocol |
The Hy3™-labeled samples and a Hy5™-labeled reference RNA sample were mixed pair-wise and hybridized to the miRCURY LNA™ microRNA Array 7th gen (Exiqon, Denmark). The hybridization was performed according to the miRCURY LNA™ microRNA Array Instruction manual using a Tecan HS4800™ hybridization station (Tecan, Austria).
|
Scan protocol |
microarray slides were scanned and stored in an ozone free environment (ozone level below 2.0 ppb) using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA)
|
Description |
Primary osteoblasts isolated from bone tissue
|
Data processing |
The quantified signals were background corrected (Normexp with offset value 10, see Ritchie et al. 2007) and normalized using the global Lowess (LOcally WEighted Scatterplot Smoothing) regression algorithm.
|
|
|
Submission date |
Oct 20, 2015 |
Last update date |
Oct 19, 2023 |
Contact name |
Júlia Perera-Bel |
E-mail(s) |
mardata-bu@researchmar.net
|
Organization name |
Hospital del Mar Research Institute
|
Department |
MARData-BU
|
Street address |
Doctor Aiguader, 88
|
City |
Barcelona |
ZIP/Postal code |
08003 |
Country |
Spain |
|
|
Platform ID |
GPL20999 |
Series (1) |
GSE74211 |
Primary Human Osteoblasts miRNA array |
|