|
| Status |
Public on Dec 21, 2015 |
| Title |
1 |
| Sample type |
RNA |
| |
|
| Source name |
Primary human osteoblasts
|
| Organism |
Homo sapiens |
| Characteristics |
cy3 data file: 1_Exiqon_19708476_S01.txt
cy5 data file: 0_Exiqon_19708476_S01.txt
tissue: Primary bone cells
condition: Osteoarthritis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using High Pure RNA Isolation kit (Roche Diagnostics, Indianapolis, USA)
|
| Label |
biotin
|
| Label protocol |
750 ng total RNA from both sample and reference was labeled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy3™/Hy5™ (Exiqon, Denmark) following the procedure described by the manufacturer
|
| |
|
| Hybridization protocol |
The Hy3™-labeled samples and a Hy5™-labeled reference RNA sample were mixed pair-wise and hybridized to the miRCURY LNA™ microRNA Array 7th gen (Exiqon, Denmark). The hybridization was performed according to the miRCURY LNA™ microRNA Array Instruction manual using a Tecan HS4800™ hybridization station (Tecan, Austria).
|
| Scan protocol |
microarray slides were scanned and stored in an ozone free environment (ozone level below 2.0 ppb) using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA)
|
| Description |
Primary osteoblasts isolated from bone tissue
|
| Data processing |
The quantified signals were background corrected (Normexp with offset value 10, see Ritchie et al. 2007) and normalized using the global Lowess (LOcally WEighted Scatterplot Smoothing) regression algorithm.
|
| |
|
| Submission date |
Oct 20, 2015 |
| Last update date |
Oct 19, 2023 |
| Contact name |
Júlia Perera-Bel |
| E-mail(s) |
mardata-bu@researchmar.net
|
| Organization name |
Hospital del Mar Research Institute
|
| Department |
MARData-BU
|
| Street address |
Doctor Aiguader, 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL20999 |
| Series (1) |
| GSE74211 |
Primary Human Osteoblasts miRNA array |
|
