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Sample GSM1911043 Query DataSets for GSM1911043
Status Public on Feb 12, 2017
Title MI+nc-7
Sample type RNA
Source name Mouse heart, myocardial infarction, 21 days
Organism Mus musculus
Characteristics strain: C57/BL6
gender: male
age: 8 weeks old
stress: myocardial infarction
tissue: heart
Growth protocol Heart tissues were collected and frozen immediately in liquid nitrogen.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed using mirVanaTM RNA Isolation Kit according to the manufacturer's instructions.
Label Biotin
Label protocol Total RNA were tailed with Poly A and then labeled with Biotin.
Hybridization protocol The labeled RNA were hybridized for 16 hr at 48°C on Affymetrix miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol The arrays were scanned by the Affymetrix Scanner 3000 (Affymetrix).
Description 21 days post ligation of left anterior descending coronary artery (LAD)
Data processing Affymetrix GeneChip Command Console software (version4.0, Affymetrix) was used to analyze array images to get raw data and then offered RMA normalization for Mm probesets. Next, Genespring software (version 12.5; Agilent Technologies) was used to proceed the following data analysis. Probes that at least 75 percent of samples in any 1 condition out of 2 conditions have flags in P were chosen for further data analysis.
Differentially expressed miRNAs were then identified through fold change as well as P value calculated using t-test. The threshold set for up- and down-regulated genes was a fold change>= 2.0 and a P value<= 0.05.
Submission date Oct 19, 2015
Last update date Feb 12, 2017
Contact name Yihua Bei
Phone +86 13816265303
Organization name Shanghai University
Department School of Life Science
Lab Regeneration Lab
Street address 133 Nanchen Road
City Shanghai
ZIP/Postal code 200444
Country China
Platform ID GPL19117
Series (1)
GSE74135 microRNA data from mice hearts of 21 days post myocardial infarction versus sham control

Data table header descriptions
VALUE RMA signal

Data table
MIMAT0000121_st 11.49746
MIMAT0004519_st 1.67355
MIMAT0000122_st 12.68874
MIMAT0004520_st 1.34434
MIMAT0016979_st 1.44215
MIMAT0000123_st 12.33562
MIMAT0000124_st 10.90077
MIMAT0004521_st 1.006157
MIMAT0016980_st 4.695799
MIMAT0000125_st 14.10266
MIMAT0004522_st 6.150665
MIMAT0000126_st 11.86299
MIMAT0004523_st 2.626274
MIMAT0000127_st 3.752149
MIMAT0000128_st 10.30543
MIMAT0000129_st 8.943307
MIMAT0000130_st 11.39951
MIMAT0004524_st 5.89801
MIMAT0000131_st 11.66756
MIMAT0016981_st 1.156535

Total number of rows: 3222

Table truncated, full table size 75 Kbytes.

Supplementary file Size Download File type/resource
GSM1911043_MI+nc-7.CEL.gz 785.4 Kb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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