|
| Status |
Public on Jul 27, 2009 |
| Title |
Fat CD4 Treg cells, repl. 3 |
| Sample type |
RNA |
| |
|
| Source name |
Abdominal adipose tissue Treg cells
|
| Organism |
Mus musculus |
| Characteristics |
Male C57Bl/6 (retired breeders 25-35 weeks old) purchased from the Jackson Laboratory. Abdominal (epidydimal) adipose tissue was cut into small pieces and digested for about 40 minutes with collagease type II. Cell suspensions were then filtered through a sieve and stromovascular fraction (SVF) was harvested after spinning.
Genetic background: Male C57Bl/6;
Age: 25-35 weeks old;
Number of mice per chip: 8-10;
Tissue: Abdominal (epidydimal) adipose tissue;
Sorting: B220-, CD11b-, CD8a-, CD4+, TCRb+, CD25+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
|
| Label |
Biotin
|
| Label protocol |
BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
|
| |
|
| Hybridization protocol |
Per Joslin Diabetes Center microarray core facility.
|
| Scan protocol |
Per Joslin Diabetes Center microarray core facility.
|
| Description |
jos3460.CEL
|
| Data processing |
RMA
|
| |
|
| Submission date |
May 18, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
|
| Relations |
| Reanalyzed by |
GSE119085 |
