|
| Status |
Public on Oct 10, 2015 |
| Title |
Control, repeat 3 |
| Sample type |
RNA |
| |
|
| Source name |
Control cells expressing the empty vector
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Cultured cancer cells
cell line: H1299
|
| Treatment protocol |
Before transfection, cells were seeded into normal growth medium at 50% confluence in six-well tissue plates. The FuGENE HD transfection reagent (Promega, Fitchburg, WI, USA) was applied according to the product manual. Briefely, the transfection complex was made by 1 μg plasmid, 3 μl FuGENE HD and 100 μl media. Six hours after the complex was added to the cells, normal culture media was used to culture cells for additional 48 h
|
| Growth protocol |
The human H1299 (p53-null) cells were maintained in DMEM medium (Gibco, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (Invitrogen) and cultured in a humidified incubator at 37 °C under 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Rneasy mini Kit from QIAGEN following the product manual.
|
| Label |
Biotin
|
| Label protocol |
A total of 200 ng small RNA was used in sample preparation with a FlashTag Biotin RNA Labeling Kit for Affymetrix GeneChip miRNA arrays (Genisphere).
|
| |
|
| Hybridization protocol |
The labeled RNA was consequently hybridized for sixteen hours to an Affymetrix GeneChip miRNA array according to the product manual.
|
| Scan protocol |
Microarrays were washed and stained in the Affymetrix Fluidics Station 450, and scanned on the Affymetrix G3000 GeneArray Scanner.
|
| Description |
miRNA expression profile
|
| Data processing |
The image files were analyzed using the Affymetrix software (Expression Console)
|
| |
|
| Submission date |
Oct 09, 2015 |
| Last update date |
Oct 10, 2015 |
| Contact name |
Jie Xu |
| E-mail(s) |
xujieletter@gmail.com
|
| Organization name |
Shanghai Institute for Digestive Diseases
|
| Department |
Shanghai Renji Hospital
|
| Lab |
State Key Laboratory for Oncogenes and Related Genes
|
| Street address |
Shandong Rd 145
|
| City |
Shanghai |
| ZIP/Postal code |
200001 |
| Country |
China |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE73876 |
Effect of mutant p53 R282W on miRNA expression |
|
