|
| Status |
Public on May 12, 2016 |
| Title |
MGG4_sphere_miRNA |
| Sample type |
RNA |
| |
|
| Source name |
MGG4 sphere cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MGG4
cell type: glioma cell line
tumor state: sphere
|
| Growth protocol |
Neurobasal medium (Invitrogen) supplemented with L-glutamine (Glutamax, Gibco), B27 supplement (Invitrogen), N2 supplement (Invitrogen), 20 ng/mL recombinant human EGF (R&D Systems), 20 ng/ml recombinant human FGF2 (R&D Systems).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Exiqon Total RNA extraction kit, on column DNASE treatment (Qiagen)
|
| Label |
Cyanine 3-pCp
|
| Label protocol |
100ng total RNA of each sample were labeled according to the Agilent miRNA Complete Labeling and Hybridization Kit.
|
| |
|
| Hybridization protocol |
Hybridization was performed at 55 °C for 20 hours while rotating with subsequent washes.
|
| Scan protocol |
Scanning was performed using high dynamic range settings on an Agilent microarray scanner. Agilent Feature Extraction Software v 10.7.3.1 was used to extract the data.
|
| Description |
MGG4s
|
| Data processing |
limma_3.24.15 on R 3.2.0 were used for cyclic-loess normalization on log transformed data
|
| |
|
| Submission date |
Oct 08, 2015 |
| Last update date |
May 13, 2016 |
| Contact name |
Tommy Beat Schlumpf |
| Organization name |
ETH Zürich
|
| Department |
D-BSSE
|
| Lab |
Paro
|
| Street address |
Mattenstrasse 26
|
| City |
Basel |
| State/province |
Basel-Stadt |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL18402 |
| Series (2) |
| GSE73846 |
Functional characterization of RNA-binding protein IMP2 in primary Glioma cell lines [array] |
| GSE73847 |
Functional characterization of RNA-binding protein IMP2 in primary Glioma cell lines |
|
