|
Status |
Public on May 12, 2016 |
Title |
RNA-Seq_mm_P21_ctrl [Sample 22] |
Sample type |
SRA |
|
|
Source name |
P21 IMP2fl/fl brain
|
Organism |
Mus musculus |
Characteristics |
strain background: C57BL6 age: Postnatal week 15 genotype/variation: C57BL6 bckgrnd mice with IMP2 floxed/floxed integrated but not crossed to Nestin Cre line tissue: brain
|
Growth protocol |
NeuroCult NSC Basal Medium (ref. 05700, Stemcell Technologies) supplemented with 10% Neurocult NSC Proliferation Supplement (ref. 05701, Stemcell Technologies) and 20ng/mL EGF
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Total RNA extraction kit Exiqon, on colum DNASE treatment (Qiagen) Illumina polyA stranded
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
processed data: RNA_brain_IMP2_KO.txt
|
Data processing |
Genomic alignment with TopHat 2.0.12, --b2-very-sensitive --library-type fr-firststrand --b2-N 1 Differential expression calling with edgeR 3.10.2 and R 3.2.0 Genome_build: mm9 Supplementary_files_format_and_content: tab-delimited textfile containing the raw readcounts, normalized cpm and gene fold-changes
|
|
|
Submission date |
Oct 08, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Tommy Beat Schlumpf |
Organization name |
ETH Zürich
|
Department |
D-BSSE
|
Lab |
Paro
|
Street address |
Mattenstrasse 26
|
City |
Basel |
State/province |
Basel-Stadt |
ZIP/Postal code |
4058 |
Country |
Switzerland |
|
|
Platform ID |
GPL19057 |
Series (2) |
GSE73845 |
Functional characterization of RNA-binding protein IMP2 in primary Glioma cell lines [HTS] |
GSE73847 |
Functional characterization of RNA-binding protein IMP2 in primary Glioma cell lines |
|
Relations |
BioSample |
SAMN04156669 |
SRA |
SRX1317337 |